THE ROLE OF QUANTITATIVE LEVEL OF INTERLEUKIN-10 IN SERUM IN THE COURSE OF HERPES ZOSTER IN ADULTS DEPENDING ON THE POLYMORPHISM OF ITS GENE

(RS1800896)

Abstract

The aim of the work – to determine the role of the dynamics of the quantitative level of interleukin-10 in the blood of patients depending on the polymorphism of its gene (rs1800896) in the course of herpes zoster in adults.

Materials and methods. The work included 50 adult patients with herpes zoster. The molecular genetics determination of the IL-10 gene (rs1800896) was carried out by the real-time polymerase chain reaction. Quantitative content of IL-10 in serum was determined by the immune enzyme method.

Results and discussion. In adult patients with TT genotype of IL-10 the development of severe course of herpes zoster (0.933 vs. 0.6, χ²=5.56, OR=9.33, 95 % CI=1.10–79.21) was more often registered and was characterized by higher (Р<0.05) level of IL-10 in serum of these patients in the dynamics of the disease. The polymorphism of gene encoding IL-10 (rs1800896) had an effect on the level of increase of this cytokine in the blood serum at the stage of hospitalization (τ=0.33, Р=0.0036), and at discharge from the hospital (τ=0.34, Р=0.0034). The genotype TT is associated with high chances of development complicated course of the disease (0.784 vs. 0.462, χ²=4.76, OR=4.23, 95 % CI=1.10–16.19), but does not influenced the spectrum of complications (Р>0.05). In the presence of complications, the level of IL-10 in the blood serum of patients at the height of the disease was higher (4.5 times, Р<0.05) than in patients with uncomplicated course of herpes zoster. In the development of the localized form of herpes zoster, the damage to the trigeminal nerve was more often recorded in patients with TT genotype (84.8 % vs. 15.2 %, χ²=6.36, Р=0.02), but dynamics of changes in the content of IL-10 in serum of these patients did not depend (p> 0.05) on localization of lesions. It was revealed that the genotype CC of IL-10 gene (rs1800896) was more commonly found in patients with recurrent herpes zoster (0.75 vs. 0.261, χ²=4.19, OR=8.5, 95 % CI=0.81-89.75). The level of IL-10 in serum of patients with relapsing disease, unlike to patients with a primary manifestation, is characterized by a less significant its increase (Р<0.05).

Conclusions. The genotype TT of IL-10 gene is associated with high chances of developing severe and complicated course of herpes zoster in adults and higher (Р<0.05) cytokine level in the dynamics of the disease. The influence of genetic polymorphism IL-10 (rs1800896) on the increase of this cytokine concentration at all stages of the disease is proved (Р<0.005). The genotype CC of IL-10 gene in adult patients with herpes zoster is associated with high chances of developing a recurrent disease (Р<0.05). The concentration of this cytokine in patients with recurrence course of herpes zoster is characterized by its moderate increase (Р<0.05). The development of localized form of the disease with a trigeminal nerve affection is associated with the genotype TT (Р<0.05). The concentration of IL-10 in serum in patients with localized forms of herpes zoster is independent of the localization of lesions (Р>0.05).