Intensity of lipid peroxidation processes and the level of markers of inflammation in the late period of combined injury in experiment
DOI:
https://doi.org/10.11603/2414-4533.2018.4.9715Keywords:
burn, skeletal trauma, combined trauma, lipoperoxidation, inflammation markers, late periodAbstract
The aim of the work: to find out the dynamics of lipid peroxidation and inflammation markers in the late period of combined trauma in the experiment.
Materials and methods. Experiments were performed on non-linear white male rats weighing 180–200 g. Under conditions of thiopental-sodium anesthesia (40 mg · kg-1) in the first experimental group, they simulated skeletal trauma by applying a pre-doses stroke to each leg that caused their closed fracture. In the second experimental group, the skin burn was modeled III A-B degree 10–11 % of the body surface – a 28 cm² copper plate was applied to the depilated surface of the back skin, pre-immersed in boiling water for 3–5 minutes. In the third group, these two injuries were combined. The control group consisted of intact animals. Animals were withdrawn from the experiment after 14, 21 and 28 days of the post-traumatic period. The content of reagents for thiobarbituric acid (TBA-active products of LPO) was determined in the liver homogenate, the serum concentration of ceruloplasmin (CP) was determined and the flow-cytometry method determined the content of proinflammatory cytokine-tumor necrotic factor-α (TNF-α).
Results and Discussion. In the period of late manifestations of traumatic illness after the application of isolated skeletal trauma, burns and their combinations, high activity of lipid peroxidation processes is noted, as evidenced by high content of liver in TBA-active products of LPO. At the same time, blood serum contains high levels of CP and TNFα, whose dynamics is oscillatory. After 14 days, the magnitude of the studied parameters is greatest after burning, after 21 days – after isolated skeletal and combined injuries. After 28 days, the activity of the inflammatory reaction goes down compared with the previous observation time. In this term, attention is drawn to the tendency to increase the serum content of CP after burn and TNFα after isolated skeletal trauma. Obviously, such a dynamics reflects the specificity of pathogenic and sanogenic relationships, aimed at adequate adaptation of the organism to the pathogenic factors of injury.
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