Morphometrical analysis of features remodeling structures of ileum at resections of different volume of liver parenchima
DOI:
https://doi.org/10.11603/2414-4533.2018.1.8865Keywords:
resection of the liver, ileum, remodeling.Abstract
The aim of the work: morphological methods to study the features of remodeling of the structures of the ileum at resection of different volumes of liver parenchyma.
Materials and Methods. The complex of morphological methods examined the ileum of 45 mature hite white male rats, which were divided into 3 groups. The first group consisted of 15 intact animals, second – 15 rats, in which 31.5 % of the liver parenchyma was removed, third – 15 animals after resection of 58.1 % of liver parenchyma. Euthanasia of rats was carried out by bloodletting in conditions of thiopental anesthesia 1 month after the beginning of the experiment. From the ileum histological preparations were made. The morphometry of thickness of the mucous, muscle, serous membranes, submucosal layers, length, width of villi, depth, width of crypts, height of cover epithelial cells, diameter of their nuclei, determined nuclear-cytoplasmic relations in epitheliocytes, relative volume of damaged epithelial cells, submucosal-mucous and submucosal-muscular indices stromal-myocytes ratio in the muscle of the organ were measured. Quantitative indicators were processed statistically.
Results and Discussion. It was established that after resection of 31.5 % of liver parenchyma morphometric indices insignificantly changed. The morphometric parameters of the ileum structures in the month after resection of 58.1 % of liver parenchyma were the most altered. At the same time, the thickness of the mucous membrane of the investigated organ with a high degree of reliability (p <0.001) decreased by 15.0 %, muscle – by 6.1 %, and the thickness of the submucosal layer increased by 7.4 %, the serous membrane – by 6.6 %. Submucosy-mucosal index increased by 27.2 %, and submucosal-muscle – by 14.4 %, compared with control morphometric indices. The length of the villi and the depth of the crypt of the ileum , the height of the epithelial cells were statistically significantly (p <0.001) decreased by 15.7 %, 15.4 % and 14.7 % respectively, while the width of the villi and crypt increased by 13.3 % and 14.2 % Nuclear-cytoplasmic ratios in the epitheliocytes of the ileum mucosa have been altered by 38.5 %, indicating a marked violation of structural cellular homeostasis. Relative volume of damaged epitheliocytes increased 28 times, and stromal-myocytic ratios in the muscular membrane increased by 22.6 %, indicating a pronounced increase in stroma in the muscle of the organ. The results of the study suggest that the removal of large volumes of liver parenchyma leads to pronounced morphological rearrangement (remodeling) of the structures of the ileum, characterized by uneven disproportionate changes in the spatial characteristics of the organ structure, violation of relations between them, vascular disorders, edema, dystrophic, necrobiotic changes in epitheliocytes, endothelial cells, stromal elements, infiltrative and sclerotic processes.
References
Avtadnilov, G.G. (2002). Osnovy kolichestvennoy patologicheskoy anatomii [Basis of quantitative pathological anatomy]. Moscow: Meditsina [in Russian].
Vishnevskiy, V.A., Yefanov, M.G., & Kazakov, I.V. (2012). Segmentarnyye rezektsyi, otdalennyye rezultaty pri zlokachestvennykh opukholyakh pecheni [Segmentar resections, long-term results in malignant liver tumors]. Ukr. Zhurnal Khirurgii – Ukrainian Journal of Surgery, 1 (16), 5-15 [in Russian].
Hnatiuk, M.S., Tatarchuk, L.V., & Yasinovskyi, O.B. (2016). Morfometrychna otsinka osoblyvostei remodeliuvania struktur dvanadtsiatypaloi kyshky pry resektsii riznykh obiemiv pechinky [Morphometric evaluation of the features of remodeling of duodenal structures during resection of different volumes of the liver]. Naukovyi visnyk Uzhhorodskoho universytetu. Seriia “Medytsyna” – Scientific Journal of Uzhhorod University. Series "Medicine", 1 (49), 3-5 [in Ukrainian].
(2003). Zahalni etychni pryntsypy eksperymentiv na tvarynakh [General ethical principles of experiments on animals]. Endokrynolohiia – Endocrinology, 8, 1, 142-145 [in Ukrainian].
Lapach, S.N., Gubenko, A.V. Babich, P.N. (2001). Statistichieskiye metody v mediko-biologicheskikh issledovaniyakh Excell [Statistical methods in medicobiological investigations Excell]. Kyiv: Morion [in Ukrainian].
Makarov, M.A., Avdyeev, S.H., & Chuchalin, A.G. (2012) Rol disfunktsyi endoteliya i regidnosti arteriy v patogeneze khronicheskoy obstruktivnoy bolezni legkikh [Role of disfunction of endothelial cell in medical and biological investigation]. Terapevticheskiy arkhiv – Theraputical archive, 3,74-80.
Sarkisov, D.S. (1998). Strukturnye osnovy adaptatsyi i kompensatsyi narushennykh funktsyy [Structural basis adaptation and compensation damage function]. Moscow: Meditsina [in Russian].
Sorochinnikov, A.G., & Dorosyevich, A.Ye. (2007). Gistologichieskaya i mikroskopicheskaya tekhnika [Histological and microscopic equipments]. Moscow: Meditsyna [in Russian].
Fiodorov, V.D., Vishnevskiy, V.A., & Nazarenko, N.A. (2007). Osnovnye oslozhneniya obshyrnykh rezektsyy pecheni i puti ikh preduprezhdeniya [The main complications of extensive liver resections and ways to prevent them]. Byull. Sibirskoy meditsyny – Bulletin of Siberian Medicine, 4, 16-24 [in Russian].
Shulhai, A.H., Tatarchuk, L.V., & Hnatiuk, M.S. (2017). Osoblyvosti remodeliuvania sudyn hemomikrotsyrkulatornoho rusla klubovoi kyshky pry rezektsii riznykh obiemiv pechinky [Remodeling peculiarities of ileum hemomicrocirculatory bed vessels at resection of different liver size]. Visnyk naukovyi doslidzen – Scientific Journal of Investigation, 4, 145-149 [in Ukrainian].
Nanashima, A., Sumida, Y., Abo, T. (2008). A modified grading system for post-hepatectomy metastatic liver сancer originating form colorectal carcinoma. J. Surg. Oncol., 98, 363-370.
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