The influence of the abdominal pressure on the dynamics of ischemically-reperfusive disorders in the experiment
DOI:
https://doi.org/10.11603/2414-4533.2018.1.8864Keywords:
intraabdominal pressure, ischemic-reperfusion syndrome, lipoperoxidation.Abstract
The aim of the work: to find out the effect of ischemic-reperfusion disorders on the intensity of processes of lipid peroxidation of blood serum, liver and the walls of the small intestine, depending on the size of intra-abdominal pressure in the experiment.
Materials and Methods. In the experiment, 78 sexually mature white male rats, Wistar weighing 200250 g, were found on a standard vivarium diet. All test animals were narcotised (sodium thiopental-40 mg.kg intraperitoneally) and a catheter measuring 20 g was injected into the abdominal cavity. In the first experimental group (24 animals), the manual insufflator was infused with atmospheric air to an intraabdominal pressure of 10 mm Hg, in the second experimental group (24 animals) 20 mm Hg, in the third experimental group (24 animals) 30 mm Hg. Art. In the control group (6 animals) air was not injected. The exposure time was 90 minutes, after which control animals and 6 animals of each experimental group were immediately withdrawn from the experiment by total blood flow to the heart. Other animals of each experimental group (6 individuals) under anesthesia were withdrawn from the experiment after 1, 3 and 7 days after the period of increased intraabdominal pressure. In the serum of blood, liver and the small intestine of test animals, the content of reagents to barbituric acid (TBK-active products) was determined, which are related to the indicative indices of the intensity of peroxide lipid oxidation.
Results and Discussion. It has been established that at the height of high intraabdominal pressure, after 90 minutes, there is a significant increase in the intensity of lipid peroxidation processes in serum, liver and wall of the small intestine, which is most pronounced with the value of intraabdominal pressure of 20 and 30 mm Hg.
The detected processes are greatly enhanced after the reduction of intraabdominal pressure to normal. Our research denuded the development of ischemic reperfusion syndrome, which is accompanied by a significant increase in the content of TBC-active products, the intensity of which increases with an increase in the level of output intraabdominal pressure. The highest increase in the content of TBC-active LPP products is noted after 3 days of reperfusion in the liver of the experimental animals (more than 5 times in the RTT 30 mm Hg), compared with the intestine (2.7 times) and serum (in 2.1 times), which is due to the increased metabolic, detoxification and immune function of the organ due to the intake of toxins of the intestinal origin. All this contributes to the activation of LPA, is an evidence of a secondary organ damage by type of reactive hepatitis and requires the devel opment of preventive measures.
References
Geldand, B.R., Protsenko, D.N., & Podachin, P.V. (2010). Sindrom intraabdominalnoy gipertenzii: sostoyanie problemy [The syndrome of intra-abdominal hypertension: the state of the problem]. Med. Alfavit. Neotlozh. Meditsina – Medical Alphabet of Emergency Medicine, 3, 36-43 [in Russian].
Gidulyanova, K.V. (2008). Vozrastnye osobennosti modulyatsii aktivnosti antioksidantbykh fermentov pri indutsiruvannom okislitelnom stresse [Age features of modulating the activity of antioxidant enzymes under induced oxidative stress]. Visnyk Kharkivskoho natsionalnoho universytetu im. V.N. Karazina. Seriia Biolohiia – Journal of Kharkiv National University by V.N. Karazin. Series Biology, 7 (814), 9-12 [in Russian].
Stefanov, O.V. (2001). Doklinichni doslidzhennia likarskykh zasobiv: metodychni rekomendatsii [Preclinical research of medicinal products: methodical recommendations]. Кyiv: Avitsenna [in Ukrainian].
Roshchin, H.H., & Kryliuk, V.O. (2008). Vnutrishnocherevnyi tysk ta syndrome abdominalnoi kompresii: pytannia etiolohii ta patohenezu [Intraperitoneal pressure and abdominal compression syndrome: issues of etiology and pathogenesis]. Ostrye i neotlozhnye sostoyaniya v praktike vracha – Acute and Urgent Conditions in the Practice of a Doctor, 1, 26-27 [in Ukrainian].
Abassi, Z., Bishara, B., Karram, T., Khatib, S., Winaver, J., & Hoffman, A. (2008). Adverse effects of pneumoperitoneum on renal function: involvement of the endothelin and nitric oxide systems. Am. J. Physiol. Regul. Integr. Comp. Physiol., 294, 842-850.
Balogh, Z., Jones, F., D'Amours, S., Parr, M., & Sugrue, M. (2004). Continuous intra-abdominal pressure measurement technique. Am. J. Surg., 188 (6), 679-184.
Del Rio, D., Stewart, A.J., & Pellegrini, N. (2005). A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr. Metab. Cardiovasc. Dis., 15, 316-328.
Lima, R.A., Schanaider, A., Santana, M.C., de Oliveira, M.G., Capelozzi, V.L., & Rocco, P.R. (2011). Developing a new experimental model of abdominal compartment syndrome. Rev. Col. Bras. Cir., 38 (6), 417-421.
Malbrain, M.L., Chiumello, D., & Pelosi, P. (2005). Incidence and prognosis of intraabdominal hypertension in mixed population of critically ill patients: а multiple-center epidemiological study. Crit. Care Med., 33 (2), 315-322.
Malbrain, M.L., & De Laet, E.L. (2009). Intra-abdominal hypertension: evolving concepts. Clin. Chest Med., 30 (1), 45-70.
Lindström, P., Wadström, J., Ollerstam, A., Johnsson, C., & Persson, A.E. (2003). Effects of increased intra-abdominal pressure and volume expansion on renal function in the rat. Nephrology Dialysis Transplantation, 18 (11), 2269-2277
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