Use of stem cells from the cord blood for the correction of violations of endogenous intoxication in rats with acute widespread peritonitis associated with mercazolyl-induced hypothyrothesis
DOI:
https://doi.org/10.11603/2414-4533.2017.3.8027Keywords:
common peritonitis, hypothyroidism, endogenous intoxication, correction, stem cells, cord blood.Abstract
The aim of the work: to study the influence of mesenchymal stem cells on the indices of endogenous intoxication in rats with acute widespread peritonitis on the background of experimental hypothyroidism.
Materials and Methods. White Wistar rats were used in the work. Hypothyroidism was modeled by the administration of Mercazolil at a dose of 25 mg / kg for 21 days. Acute common peritonitis was modeled by injecting 0.5 ml of 10 % filtered fecal suspension into the abdominal cavity of the animals under study. Stem cells were obtained from umbilical cord blood of pregnant females on the 21–24th day of pregnancy. Evaluation of the viability of the obtained cells was carried out using the test for inclusion of the supra-red dye by staining with 0.1 % trypan blue. The suspension obtained mononuclear cells were injected intraperitoneally in a dose of 0.5 ml (number of cells administered dose – 0.9–1.0 × 108) immediately after the simulation common acute peritonitis. determination of the average molecular weight was conducted, leukocyte and erythrocyte indices intoxication activity of cathepsin D.
Results and Discussion. The research found that the presence of hypothyroidism in experimental animals, which simulated an acute common fecal peritonitis, accompanied by a more pronounced than in euthyroid rats, an increase of endotoxemia indicators – middle molecules, erythrocyte and leukocyte index of intoxication, and slow dynamics of their normalization. Phase changes observed activity of cathepsin D. The use of cord blood stem cells is accompanied by a significant decrease in endogenous toxicity in animals with experimental peritonitis common amid Mercazolilum-induced hypothyroidism. Deficiency of iodine-containing thyroid hormones accompanied by a significant increase in the indicators characterizing the syndrome of endogenous intoxication compared to eutireoid animals. This may be one of the reasons for the development of multiple organ failure in these conditions. The use of stem cell cord blood is accompanied by a significant decrease in endogenous intoxication in experimental animals with generalized peritonitis in the background of mercazolil-induced hypothyroidism.
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