DYNAMICS OF CERULOPLASMIN CONTENT IN BLOOD SERUM UNDER CONDITIONS OF MECHANICAL TRAUMA OF VARIOUS LOCALIZATION SEPARATELY AND IN COMBINATION WITH ACUTE BLOOD LOSS
DOI:
https://doi.org/10.11603/2414-4533.2026.1.16102Keywords:
mechanical trauma, blood loss, ceruloplasminAbstract
The aim of the work: to determine the dynamics of ceruloplasmin (CP) content in blood serum under conditions of simulated mechanical trauma of various localizations, separately and in combination with acute blood loss.
Materials and Methods. In mature male Wistar line rats under anesthesia (sodium thiopental, 40 mg/kg), traumatic brain injury (TBI), blunt abdominal trauma (BAT), and skeletal trauma (ST) were simulated. Additionally, rats with these injuries administered acute blood loss in an amount of 1.5 % of their body weight. After 3, 7, 14, 21, and 28 days of the post-traumatic period, the CP content was determined in the blood serum of the experimental rats.
Results. It was established that, regardless of the location of the mechanical injury, the CP content in rats increased 3 days after mechanical trauma of various locations. Subsequently, over the course of 7–28 days, the indicator gradually decreased with a period of temporary re-growth after 21 days. At all-time points after injury, the concentration of CP was significantly higher in rats with BAT than in other experimental groups. Under conditions of additional acute blood loss, the dynamics of CP content in blood serum was similar, but the amplitude of disturbances increased. After TBI and acute blood loss, the indicator was significantly higher compared to rats with monotrauma at all stages of the post-traumatic period, under conditions of BAT and acute blood loss, respectively, 7, 14, 21, and 28 days after the injury, and after modeling of ST – 3, 14, 21, and 28 days later. However, in rats with abdominal injuries and acute blood loss, the indicator was significantly higher compared to other experimental groups.
Conclusions. Mechanical injuries of different localizations vary significantly in terms of CP formation and respond differently to acute blood loss complications. The obtained results emphasize the importance of determining CP as a biomarker of inflammation for assessing and understanding the inflammatory process, which is of practical importance in developing strategies for systemic correction.
Received: 12.01.2025 | Revised: 28.01.2026 | Accepted: 23.02.2026
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