THE INFLUENCE OF SPLENECTOMY ON THE INTENSITY OF LIPID PEROXIDATION PROCESSES AND THE ACTIVITY OF ENZYMATIC AND GLUTATHIONE LINKS OF ANTIOXIDANT PROTECTION OF THE LIVER IN THE EXPERIMENT
DOI:
https://doi.org/10.11603/2414-4533.2025.4.15752Keywords:
splenectomy, laparotomy, lipid peroxidation, superoxide dismutase, catalase, experimentAbstract
The aim of the study: to determine the influence of splenectomy on the intensity of lipid peroxidation processes and the activity of enzymatic and glutathione links of antioxidant protection of the liver in the experiment.
Materials and Methods. The experiment was performed on 54 healthy mature male Wistar line rats weighing 200–220 g. The experiments were conducted under thiopental sodium anesthesia at a dose of 40 mg·kg-1. Rats that were only anesthetized were placed in control group 1. In control group 2, laparotomy was performed under thiopental sodium anesthesia. In the experimental group, splenectomy was performed. After 1, 7, 14, and 28 days, the content of diene and triene conjugates (DC, TC) and thiobarbituric acid reagents (TBK-active products of lipid peroxidation) as well as superoxide dismutase (SOD activity) and catalase activity were determined. The antioxidant-prooxidant index (API) was calculated based on the ratio of catalase activity to TBK-active products of lipid peroxidation.
Results. It was established that modeling only laparotomy compared to control caused an increase in the content of TBK-active products of LPO during the first 7 days of the experiment, DC and TC during 1, 7, and 28 days of the experiment. After 14 days, the content of the studied LPO indicators normalized, but by the 28th day, a repeated increase in the content of primary LPO products in the liver was noted: DC and TC. After laparotomy, SOD activity in the liver did not differ significantly from the control group during the 14 days of the experiment, but increased after 28 days, indicating secondary prooxidative disorders in the liver in the late period after the inflicted injury. However, catalase activity throughout the experiment was elevated with two periods of maximum growth – 1 and 14 days after the post-laparotomy period. The API value also increased up to 14 days and reached its maximum level, which clearly indicates the dominance of antioxidant mechanisms during this period. Against the background of splenectomy, the studied LPO indicators at all studied periods of the experiment were significantly higher compared to rats that underwent only laparotomy. The maximum increase in LPO processes was observed 7 days after the surgical intervention. During this period, the greatest decrease in API and a compensatory increase in SOD activity were also observed. By the end of the experiment, the indicators had returned to the norm, but the LPO indicators remained significantly higher, and the antioxidant protection indicators lower, compared to rats that had undergone only laparotomy.
Conclusions. Laparotomy and spleenectomy contribute to secondary damage to internal organs, in particular the liver, due to the intensification of LPO processes and a decrease in the activity of the enzymatic link of antioxidant protection.
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