INFLUENCE OF ACUTE BLOOD LOSS ON THE INTENSITY OF LIPID PEROXIDATION PROCESSES IN THE KIDNEY OF RATS WITH TRAUMATIC BRAIN INJURY, BLUNT ABDOMINAL TRAUMA AND SKELETAL TRAUMA
DOI:
https://doi.org/10.11603/2414-4533.2025.1.15187Keywords:
traumatic brain injury, blunt abdominal trauma, skeletal trauma, kidney, oxidative stressAbstract
The aim of the work: to determine the influence of acute blood loss on the intensity of lipid peroxidation processes in the kidney of rats with traumatic brain injury, blunt abdominal trauma, and skeletal trauma.
Materials and Methods. In experiments on mature male Wistar line rats under conditions of thiopental sodium anesthesia, traumatic brain injury (TBI), blunt abdominal trauma (BAT), and skeletal trauma (ST) were modeled. In some experimental groups, on the background of mechanical trauma of different localization, acute blood loss was additionally induced in the amount of 1,5 % of body weight. The rats were taken out of the experiment after 3, 7, 14, 21, and 28 days of the post-traumatic period. The content of thiobarbituric acid reagents (TBA-active products) was determined in the kidney homogenate extract.
Results. The modeling of TBI, BAT and ST caused an increase in lipid peroxidation processes in the kidney, as evidenced by the accumulation of TBA-active lipid peroxidation products in the organ with a maximum within 7–21 days after TBI and two periods of increase – after 7 and 21 days of the experiment. After 3, 21, and 28 days of post-traumatic period, the index significantly prevailed in the conditions of BAT. Complication of modeled injuries with acute blood loss of 1,5 % of body weight during all periods of observation was accompanied by a statistically significant increase in the intensity of lipid peroxidation processes in the kidney compared with stand-alone injuries. The content of TBA-active lipid peroxidation products in the kidney under conditions of TBI and ST changed in a single phase with a maximum after 7 days of post-traumatic period, under conditions of BAT – continued to grow in a biphasic manner with maximums after 7 and 21 days of experiment. After 14 days, the index prevailed in the conditions of TBI, after 21 and 28 days – in the conditions of BAT. The degree of increase in the content of TBA-active lipid peroxidation products in the kidney in the conditions of BAT and ST during the post-traumatic period was not significantly different, but in the conditions of TBI after 14 and 21 days was significantly higher, indicating a greater pathogenic role of acute blood loss in these periods in the systemic manifestations of traumatic brain injury.
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