THE EFFECT OF CRANIO-SKELETAL TRAUMA ON THE CONTENT OF 8-ISOPROSTANE IN BLOOD SERUM IN RATS OF DIFFERENT AGES AND THE EFFECTIVENESS OF CELL THERAPY

Authors

DOI:

https://doi.org/10.11603/2414-4533.2024.4.15071

Keywords:

traumatic brain injury, combined trauma, oxidative stress, antioxidant system, 8-isoprostane, age

Abstract

The aim of the work: to determine the effect of cranio-skeletal trauma (CST) on the serum level of 8-isoprostane in rats of different ages and to evaluate the effectiveness of cell therapy.

Materials and Methods. The study was conducted on white male Wistar line rats of three age groups: immature rats aged 100-120 days, mature rats aged 6-8 months, and old rats aged 19-23 months. Rats of each age group were modeled with CST under thiopental sodium anesthesia. For the purpose of correction, a suspension of cryopreserved neuroblast cells (NBC) was injected intravenously separately and in combination with intraperitoneal injection of mesenchymal stem cells (MSC). Control rats from each age group were administered only sodium thiopental anesthesia, and instead of cells, saline solution in an equivalent dose. After 14 days of the experiment, the content of 8-isoprostane was determined in the blood serum.

Results. The modeling of CST in rats of different age groups after 14 days of post-traumatic period compared to the control was accompanied by a significant increase in the intensity of lipid peroxidation processes, as evidenced by a statistically significant increase of 8-isoprostane in the blood serum. The degree of index growth was the same among rats of different age groups and proportional to its value in the control, where it significantly prevailed in immature rats. Isolated injection of NBC and its combination with MSC caused a decrease in the intensity of lipid peroxidation processes compared to rats without correction and in the groups of immature and mature rats was accompanied by a statistically significant decrease in the content of 8-isoprostane in the blood serum. The degree of decrease in the studied index was statistically significantly higher in the group of mature rats, especially after combined NBC and MSC therapy.

Conclusions. The combined injection of NBC and MSC is accompanied by a decrease in the intensity of oxidative stress in rats with CST, the effectiveness of which is higher in mature rats compared to rats of other age groups, which should be taken into account in the complex therapy of experimental trauma.

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Published

2025-01-10

How to Cite

SUSHKO Y. І. (2025). THE EFFECT OF CRANIO-SKELETAL TRAUMA ON THE CONTENT OF 8-ISOPROSTANE IN BLOOD SERUM IN RATS OF DIFFERENT AGES AND THE EFFECTIVENESS OF CELL THERAPY. Hospital Surgery. Journal Named by L.Ya. Kovalchuk, (4), 55–61. https://doi.org/10.11603/2414-4533.2024.4.15071

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Section

EXPERIMENTAL INVESTIGATIONS