DYNAMICS OF SUPEROXIDE DISMUTASE AND CATALASE ACTIVITY OF THE LIVER OF RATS OF DIFFERENT AGE IN CASE OF EXPERIMENTAL CRANIOSKELETAL INJURY
DOI:
https://doi.org/10.11603/2414-4533.2024.3.14924Keywords:
traumatic brain injury, multiple trauma, liver, oxidative stress, antioxidant activity, superoxide dismutase, catalaseAbstract
The aim of the work: to discover the dynamics of superoxide dismutase (SOD) and catalase activity of the liver of rats of different age in case of experimental cranioskeletal injury.
Materials and Methods. Cranioskeletal trauma (CST) was modelled in rats of three age groups (sexually immature, sexually mature, old) under thiopental sodium anesthesia. Rats were removed from the experiment under anesthesia after 1, 3, 7, 14, 21 and 28 days of the post-traumatic period. SOD and catalase activity was determined in the liver homogenate.
Results. It was identified that, regardless of age, under the influence of CST in the liver of experimental rats, SOD and catalase activity significantly decreases with a minimum after 3-14 days of the post-traumatic period and a subsequent increase, which reaches the control level only in sexually immature rats by 28 days. However, the degree of decrease of the studied indicators is significantly greater in old rats: in SOD activity after 28 days (by 24.0% compared to sexually immature rats and by 23.3% compared to sexually mature rats), in catalase activity – starting from the 3rd day of the experiment. The lowest degree of decrease in SOD and catalase activity was observed in sexually immature rats. The obtained results indicate a systemic pro-oxidant effect of the modelled injury on organs which are distant from the site of direct injury, as well as a decrease in the antioxidant capacity of the liver of rats with increasing age.
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