THE INFLUENCE OF INCREASED INTRA-ABDOMINAL PRESSURE ON THE INTENSITY OF LIPID PEROXIDATION PROCESSES IN THE LIVER IN RATS OF DIFFERENT AGE GROUPS
DOI:
https://doi.org/10.11603/2414-4533.2024.3.14923Keywords:
intra-abdominal pressure (IAP), oxidative stress, liver, ageAbstract
The aim of the work: to determine the influence of increased intra-abdominal pressure on the intensity of lipid peroxidation processes in the liver in rats of different age groups.
Materials and Methods. The experiments were performed on 70 white male Wistar line rats of two age groups: mature rats aged 6-8 months and weighing 180-200 g and old rats aged 19-23 months and weighing 300-320 g. Under thiopental sodium anesthesia, the abdominal cavity was catheterized and atmospheric air was inflated with a manual insufflator to an intra-abdominal pressure up to 20 mm Hg. The exposure time was 60 minutes. The rats were taken out immediately after deflation, as well as after 1, 3, and 7 days. The content of lipid peroxidation products: diene and triene conjugates (DC, TC) and reagents to thiobarbituric acid (TBA-active lipid peroxidation products) were determined in a 10% liver homogenate extract.
Results. It was found that a 60-minute increase in intra-abdominal pressure to 20 mm Hg caused an increase in the processes of lipid peroxidation in rats of different age groups. The maximum increase in the liver content of primary and secondary lipid peroxidation products was observed at the 1st day after deflation of the abdominal cavity, which remained at the same level until the 3rd day of the experiment. After 7 days, the value of the studied indicators of lipid peroxidation in mature rats reached the control level, but in old rats the content of DC and TC in the liver continued to be significantly higher. The degree of growth of these indicators in old rats after the 1st and 3rd days of the experiment was statistically significantly higher than in mature rats, but after 7 days it became almost the same. The obtained result indicates that liver lipid peroxidation disorders caused by an increase in IAP up to 20 mm Hg for the duration of 1 hour in rats of different age groups are within the limits of homeostatic regulation, but in old rats it requires more time for rehabilitation.
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