PECULIARITIES OF LIPID PEROXIDATION PROCESSES IN THE LIVER OF RATS WITH CRANIO-CEREBRAL TRAUMA, BLUNT ABDOMINAL TRAUMA AND SKELETAL TRAUMA
DOI:
https://doi.org/10.11603/2414-4533.2024.3.14922Keywords:
cranio-cerebral trauma, blunt abdominal trauma, skeletal trauma, liver, oxidative stressAbstract
The aim of the work: to compare lipid peroxidation disorders in the liver in the dynamics of standardized models of cranio-cerebral trauma (CCT), blunt abdominal trauma (BAT) and skeletal trauma (ST).
Materials and Methods. We modeled cranio-cerebral trauma (CCT), blunt abdominal trauma (BAT), and skeletal trauma (ST) in sexually mature male Wistar line rats under thiopental sodium anesthesia. Rats were taken out of the experiment in the conditions of anesthesia after 3, 7, 14, 21 and 28 days of the post-traumatic period. The content of thiobarbituric acid reagents (TBA-active products) was determined in the liver homogenate extract.
Results. Under the conditions of modeling CCT, BAT and ST, oxidative stress occurs at the systemic level in the body of experimental rats, which is manifested by an increase in the content of TBA-active lipid peroxidation products in the liver during 28 days of the posttraumatic period, which only in rats with ST normalizes by the end of the experiment. In the dynamics of the post-traumatic period, individual amplitude and time fluctuations in the content of TBA-active lipid peroxidation products in the liver are characteristic of each trauma localization: in the case of CCT, the index increases up to the 14th day, and then decreases up to the 28th day, not reaching the control level; in case of BAT, the index changes fluctuatingly with the first increase after 7–14 days and the second – after 28 days of the posttraumatic period; in case of ST, two periods of growth of the index are noted: the first – after 3 days of the posttraumatic period and the second with a smaller amplitude – after the 21st day. After 7, 14, and 28 days of posttraumatic period, the content of TBA-active lipid peroxidation products is significantly higher in the background of BAT modeling, which is associated with direct mechanical impact on the abdominal cavity; after 3 days, the index is also dominant in the conditions of ST, after 21 days – after CCT.
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