INTEGRAL EVALUATION OF SYSTEMIC MANIFESTATIONS OF TRAUMATIC DISEASE UNDER CONDITIONS OF CRANIOSKELETAL TRAUMA IN RATS OF DIFFERENT SEXES
DOI:
https://doi.org/10.11603/2414-4533.2024.2.14849Keywords:
craniocerebral trauma, skeletal trauma, male rats, female rats, heart, traumatic disease, lipid peroxidation, inflammation, tension of regulatory systemsAbstract
The aim of the work: to determine the effect of cranioskeletal trauma on the dynamics of systemic manifestations markers of TD (traumatic disease) in rats of different sexes.
Materials and Methods. The experiments were performed on white Wistar line rats weighing 200-220 g of different sexes. Rats were divided into control (6 female and 6 male rats) and experimental groups (45 rats of different sexes). Under conditions of thiopental sodium anesthesia at a dose of 40 mg∙kg-1, cranioskeletal trauma was modeled in the experimental group. Control rats of different sexes were only injected with anesthesia. In rats of the experimental group, after 1, 3, 7, 14 and 28 days of the post-traumatic period, in order to assess the degree of activation of the central mechanisms of heart rate regulation, a cardiac intervalogram was recorded and the index of regulatory mechanisms tension was calculated. Then rats were euthanized and the content of thiobarbituric acid reactive substances was determined in the heart homogenate extract, and the content of ceruloplasmin was determined in the blood serum.
Results. Under conditions of experimental cranioskeletal trauma, the intensity of lipid peroxidation processes in the heart of males significantly increased compared to females. This was evidenced by a greater degree of increase in the content of thiobarbituric acid reactive substances in the heart after 7, 14, and 28 days of posttraumatic period. In males, experimental trauma was accompanied by a prolonged proinflammatory reaction with a statistically significant increase in serum ceruloplasmin content compared to females, starting from the 3rd day of the posttraumatic period. Under conditions of the modeled trauma, there was an increase in the centralization of heart rate control, as indicated by an increase in the index of tension of regulatory systems, the value of which in rats of different sexes fluctuated in antiphase: in males it increased after the 1st and 14th days of the experiment, in females - after the 3rd day with a subsequent decrease. The index after 14 and 28 days of the experiment in females was statistically significantly lower than in males.
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