FEATURES OF THE DEVELOPMENT OF OXIDATIVE STRESS IN THE LIVER OF DIFFERENT SEXES RATS WITH METABOLICALLY ASSOCIATED STEATOTIC LIVER DISEASE AND ITS CORRECTION WITH INOSITOL
DOI:
https://doi.org/10.11603/2414-4533.2024.2.14847Keywords:
oxidative stress, liver, metabolically associated steatotic liver disease, lipid peroxidation, antioxidant system, morphological changes, rats, sexAbstract
The aim of the work: to investigate the changes in the products of postoxidation of lipids, the activity of antioxidants and morphological changes in the liver of rats of different sexes with metabolically associated steatotic liver disease (MASLD) and their correction with inositol.
Materials and Methods. Experiments were performed on 96 rats of different sexes. The animals were divided into 4 groups - control, MASLD 4 months, MASLD 4 months + myo-inositol, MASLD 4 months + D-chiro-inosito. In the MASLD groups, 4 animals consumed a 20% fructose solution instead of water for 4 months. After 2 months from the beginning of the experiment, for 2 months, the rats of the MASLD group 4 months + Myo-inositol were intragastrically injected with myo-inositol (400 mg/kg), the MASLD group 4 months + D-chiro-inositol – D-chiro-inositol (30 mg/ kg). Liver samples were taken for morphological examination, and the content of diene and triene conjugates (DC, TC), Schiff's bases (SB), TBA-active products (TBA-ap), superoxide dismutase and catalase activity (SOD, CAT) was determined in its homogenates.
Results and Discussion. Males of the control group, compared to females, have higher DC, TC, TBA-ap, and lower SOD. In animals with MASLD, DC, TC, SB, TBA-ap, SOD, and CAT increased sharply, and DC, TC remained larger in males, and SB - in females. Myo-inositol caused less accumulation of DC, TC, SB, TBA-ap, SOD and CAT in males and females, and only CAT was lower in females. D-chiro-inositol also led to a lower accumulation of DC, TC, SB, TBA-ap, SOD, and CAT in males and females, but females had higher DC, and males – TBCap, CAT. Compared to myo-inositol, D-chiro-inositol showed a greater effect on SOD in females. Similarly, the investigated inositol improved the morphological condition of the liver, reducing steatoepatosis.
References
Wong VWS, Adams LA, de Lédinghen V, Wong GLH, Sookoian S. Noninvasive Biomarkers in NAFLD and NASH – Current Progress and Future Promise. Nat. Rev. Gastroenterol. Hepatol. 2018; 15:461–478.
Xie C, Liu K, Xie Y, Liu S, Ji B. Metabolism-related signalling pathways involved in the pathogenesis and development of metabolic dysfunction-associated steatotic liver disease. Clin. Res. Hepatol. Gastroenterol. 2024; 48(2):102264.
Bedossa P. Pathology of Non-Alcoholic Fatty Liver Disease. Liver Int. 2017; 37:85–89.
Martín-Fernández M, Arroyo V, Carnicero C, Sigüenza R, Busta R, Mora N, Antolín B, Tamayo E, Aspichueta P, Carnicero-Frutos I, Gonzalo-Benito H, Aller R. Role of Oxidative Stress and Lipid Peroxidation in the Pathophysiology of NAFLD. Antioxidants (Basel). 2022; 11(11):2217.
Diehl AM, Day C. Cause, Pathogenesis, and Treatment of Nonalcoholic Steatohepatitis. N. Engl. J. Med. 2017;377:2063–2072.
Kostyuk OA, Denefil OV, Holovata TK. Changes in biochemical parameters in the blood of high-and low-emotional rats with ethanol hepatosis. Medical and clinical chemistry. 2015;20(3): 125–132. in Ukrainian.
Bevilacqua A, Dragotto J, Giuliani A, Bizzarri M, Bevilacqua A. Myo-inositol and D-chiro-inositol (40:1) reverse histological and functional features of polycystic ovary syndrome in a mouse model. Journal of Cellular Physiology. 2019;234: 9387–9398.
Vlizlo VV, Fedoruk RS, Ratych IB. Laboratory research methods in biology, animal husbandry and veterinary medicine: a handbook. Lviv: SPOLOM, 2012;764. in Ukrainian.
Tkach SM, Cheverda TL. Non-alcoholic steatohepatitis in patients with endocrine pathology. Literature review. KEtaEH [Internet]. 2021;2:82–93. http://jcees.endocenter.kiev.ua/article/view/235587 in Ukrainian.
Delli Bovi AP, Marciano F, Mandato C, Siano MA, Savoia M, Vajro P. Oxidative Stress in Non-alcoholic Fatty Liver Disease. An Updated Mini Review. Front Med (Lausanne). 2021;8:595371.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 О. В. СОПЕЛЬ, О. В. ДЕНЕФІЛЬ
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish their work in Hospital Surgery. Journal by L. Ya. Kovalchuk agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
