SEX PECULIARITIES OF OXIDATIVE STRESS DEVELOPMENT IN THE LIVER UNDER THE INFLUENCE OF CRANIOSKELETAL TRAUMA AND ITS CORRECTION
DOI:
https://doi.org/10.11603/2414-4533.2024.2.14846Keywords:
craniocerebral trauma, skeletal trauma, male rats, female rats, liver, oxidative stress, catalase, superoxide dismutase, 2-ethyl-6-methyl-3-hydroxypyridine succinateAbstract
The aim of the work: to determine the effect of experimental cranioskeletal trauma on the development of oxidative stress in the liver of rats of different sexes and to evaluate the effectiveness of 2-ethyl-6-methyl-3-hydroxypyridine succinate in correction of the detected disorders.
Materials and Methods. In the experimental setup, 78 white Wistar line female rats and 78 white male rats, each weighing 200-220 g, were involved. Under conditions of thiopental sodium anesthesia, cranioskeletal trauma was modeled in experimental group 1, and in experimental group 2, cranioskeletal trauma in rats was corrected with a solution of 2-ethyl-6-methyl-3-hydroxypyridine succinate. Control rats of different sexes were only injected with anesthesia. The rats of experimental group 1 were withdrawn from the experiment on day 1, 3, 7, 14 and day 28 after trauma modeling. The rats of experimental group 2 were withdrawn from the experiment after 7, 14, and 28 days of the posttraumatic period. Liver was taken for the investigation, catalase activity and the content of thiobarbituric acid reactive substances were determined in the homogenate. According to their ratio, the antioxidant-prooxidant index was calculated, the value of which indicates the development of oxidative stress in the liver.
Results. Under the influence of the modeled trauma in male rats, the maximum decrease in the value of the antioxidant-prooxidant index in the liver was observed after 3 days of the experiment, in female rats - after 7 days, with a subsequent increase, which in rats of both sexes did not reach the control level until 28 days. In all terms of the post-traumatic period, the value of the index was statistically significantly higher in female rats compared to male rats. Under the influence of 2-ethyl-6-methyl-3-hydroxypyridine succinate, compared to rats without correction, the disturbances of the liver antioxidant-proliferative index were less, which was statistically significant in female rats after 7 days of the experiment, and in male rats after 7, 14 and 28 days of the experiment. Therefore, the intensity of prooxidant mechanisms in response to trauma in the liver of male rats is higher than in female rats, but the effectiveness of 2-ethyl-6-methyl-3-hydroxypyridine succinate in male rats is higher.
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