Structural changes in the heart, lungs and kidneys with peritonitis in animals with the metabolic syndrome
DOI:
https://doi.org/10.11603/2414-4533.2022.4.13611Keywords:
peritonitis, metabolic syndrome, heart, lungs, kidneysAbstract
The aim of the work: to establish the peculiarities of morphological changes of internal organs in metabolic syndrome on the background of peritonitis in the experiment.
Materials and Methods. The study was conducted on 16 sexually mature non-linear white male rats. Acute widespread peritonitis was modeled by injecting 10 % filtered fecal suspension into the abdominal cavity at a dose of 0.5 ml per 100 g of animal weight according to V. A. Lazarenko. and co-authors (2008). Male rats of the control group were fed a normal diet. Base group male rats (n=8) were fed a high-fat diet (over 60 % of energy from fat) for 16 weeks. Histological sections were prepared on an Amos AMR-400 rotary microtome with a thickness of 3-5 μm (at least two sections for each histological slide), stained with hematoxylin and eosin. Research and photo-registration of the preparations was carried out using an Eclipse Ci-E microscope (Japan) with a Sigeta M3CMOS 14000 digital camera at various magnifications x100, x200, x400.
Results and Discussion. In animals with metabolic syndrome, after simulating peritonitis in the early periods, pronounced (progressive and increasing) structural changes in the myocardium and lungs were visualized, which are manifested by dystrophic, dystrophic-necrotic changes, inflammatory infiltration of the stroma, and acute progressive circulatory disorders in comparison with animals without metabolic syndrome. In our studies, the kidneys of animals with structural manifestations similar to those of animals without metabolic syndrome turned out to be less vulnerable.
References
Lazarenko, V.A., Lipatov, V.A., Blinkov, Yu.Yu., & Skorikov, D.V. (2008). Eksperimentalnaya model rasprostranennogo kalovogo peritonita [Experimental model of widespread fecal peritonitis]. Chelovek i yego zdorove – Man and His Health, 4, 128-132 [in Russian].
Bagrіi, M.M., Dіbrova, V.A., Popadinets, O.G., & Grishchuk, M.І. (2016). Metodiki morfologіchnikh doslіdzhen: monografіya [Methods of morphological research: monograph]. Vіnnytsia: Nova knyha [In Ukrainian].
(2019). Modelyuvannya metabolichnoho syndromu riznoho henezu v eksperymentalnykh tvaryn: metodychni rekomendatsiyi [Modeling of metabolic syndrome of different genesis in exper-imental animals :guidelines]. Kharkiv: Natsionalna Akademiya Medychnykh Nauk Ukrayiny [in Ukrainian].
Nijhuis, J., Rensen, S.S., Slaats, Y., Van Dielen, F.M., Buurman, W.A., & Greve, J.W.M. (2009). Neutrophil activation in morbid obesity, chronic activation of acute inflammation. Obesity, 17(11), 2014-2018.
Prasad, N.B.G., & Reddy, K.B. (2016). A study of acute peritonitis: evaluation of its mortality and morbidity. International Surgery Journal, 3(2), 663-668.
Samson, S.L., & Garber, A.J. (2014). Metabolic syndrome. Endocrinology and Metabolism Clinics, 43(1), 1-23.
Utzolino, S., Ditzel, C.M., Baier, P.K., Hopt, U.T., & Kaffarnik, M.F. (2014). The obesity paradox in surgical intensive care patients with peritonitis. Journal of Critical Care, 29(5), 887-e1.
Zyromski, N.J., Mathur, A., Pitt, H.A., Lu, D., Gripe, J.T., Walker, J.J., ... & Swartz-Basile, D.A. (2008). A murine model of obesity implicates the adipokine milieu in the pathogenesis of severe acute pancreatitis. American Journal of Physiology-Gastrointestinal and Liver Physiology, 295(3), 552-558.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Hospital Surgery. Journal named by L.Ya. Kovalchuk
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish their work in Hospital Surgery. Journal by L. Ya. Kovalchuk agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
