The effect of castration on the development of epinephrine damage to the heart in rats with chronic hypodynamic stress
DOI:
https://doi.org/10.11603/2414-4533.2022.4.13607Keywords:
heart, adrenalin, oxidative stress, morphological changes, hypodynamia, castrationAbstract
The aim of the work: to evaluate the effect of castration on the development of oxidative processes and morphological changes in the heart of rats that have chronic stress, in epinephrine heart damage (EHD).
Materials and Methods. Experiments were performed on 120 white male Wistar rats. All animals were divided into two series: 1 – stress, 2 – castration and stress. To reproduce EHD, rats were injected once intraperitoneally with a 0.18 % solution of adrenaline hydrotartrate at the rate of 0.5 mg/kg of weight (Pharmaceutical company "Darnytsia", Ukraine). The development of oxidative stress and morphological changes in the control heart were evaluated 1, 3, 7, 14 and 28 days after the injection of adrenaline.
Results and Discussion. Castration and stress in animals of the II series, compared with the I series of rats that were only exposed to stress, caused a decrease in the products of lipid peroxidation, but a significant increase in oxidatively modified proteins. Superoxide dismutase activity in the II series increased, and catalase activity significantly decreased. Adrenaline led to the greatest accumulation of TBA-active products: in animals that were stressed – after 14 days, and castration and stress – after 1 day. The degree of accumulation of oxidatively modified proteins is more pronounced in animals that have experienced stress, but the absolute values are significantly greater in rats that have been previously castrated. In the first series of animals, after injection of adrenaline, superoxide dismutase increased, but catalase activity decreased. In the II series of animals, the activity of antioxidants increased, but remained significantly lower, compared to the indicators of the I series of rats. Morphological changes indicated significant damage to the myocardium and vascular wall of both series of animals.
Castration causes grater disturbances of oxidative processes in the heart of rats with chronic hypodynamic stress, during the development of adrenaline damage to the myocardium.
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