THE ROLE OF LIPID PEROXIDATION PROCESSES IN THE DEVEPOLMENT OF IMPAIRED BILE PRODUCTION UNDER THE INFLUENCE OF SKELETAL TRAUMA COMPLICATED BY ACUTE BLOOD LOSS IN COMBINATION WITH MECHANICAL DAMAGE OF SKIN, AND PRP-THERAPY EFFECTIVENESS
DOI:
https://doi.org/10.11603/2414-4533.2022.2.13172Keywords:
mechanical damage of skin, skeletal trauma, blood loss, liver, lipid peroxidation, bile acids, PRP-therapyAbstract
The aim of the work: to establish the role of lipid peroxidation processes in the devepolment of impaired bile production under the influence of skeletal trauma complicated by acute blood loss in combination with mechanical damage of skin, and PRP-therapy effectiveness.
Materials and Methods. The experimental studies were conducted on 186 nonlinear white male rats weighing 180–200 g, which were divided into five groups: the control and four experimental ones. The control group consisted solely of the intact animals exposed to general anesthesia. In the first experimental group, the animals were simulated a mechanical damage of skin, the rats of the second experimental group were subjected to a skeletal trauma complicated by acute blood loss, the third experimental group had both these lesions combined. In the fourth
experimental group, the PRP-therapy was applied using allogeneic platelet-rich plasma injections to the animals with combined trauma. After 3, 7, 14, 21 and 28 days of the posttraumatic period, the common bile duct was catheterized and the bile was collected from the control and experimental groups. The concentration of total bile acids in the bile was subsequently determined in all bile samples. The level of thiobarbituric acid reactive substances (TBARS) was measured in liver homogenate.
Results and Discussion. The conducted studies indicated the enhancement of lipid peroxidation processes in the liver of experimental animals as a result of mechanical damage of skin, skeletal trauma complicated by acute blood loss and the combined trauma model. It was evidenced by the concomitant increase in TBARS values, which did not reach the control level down to the end of experiment. The dynamic of studied indicator was undulant, as the first maximum occurred after 7 days of the posttraumatic period and the second one – after 21 days. The concentration of total bile acids in the bile decreased up to the 7th day, remained at the same level until the 21st day, and, subsequently, increased up to 28th day, though not reaching control values. It was found that a more severe trauma severity led to the greater increase in lipid peroxidation activity as well as more impaired hepatic bile acid synthesis. The administration of the PRP-therapy contributed to a statistically significant decreased TBARS levels in the liver starting from the 21st day of the experiment as well as the increased total bile acids concentration in the bile of rats subjected to combined trauma model as compared to the animals without corrective medication.
The infliction of mechanical damage to skin leads to the enhancement of lipid peroxidation processes in the liver, which results in the obstruction of hepatic bile acid synthesis by endoplasmic reticulum membranes. The intradermal administration of allogeneic platelet-rich plasma injections reduces the systemic manifestations of traumatic disease, which is evidenced by the inhibition of hepatic lipid peroxidation as well as the regression of impaired functionality of endoplasmic reticulum membranes.
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