THE ROLE OF LIPID PEROXIDATION PROCESSES IN THE DEVEPOLMENT OF IMPAIRED BILE PRODUCTION UNDER THE INFLUENCE OF SKELETAL TRAUMA COMPLICATED BY ACUTE BLOOD LOSS IN COMBINATION WITH MECHANICAL DAMAGE OF SKIN, AND PRP-THERAPY EFFECTIVENESS
DOI:
https://doi.org/10.11603/2414-4533.2022.2.13172Keywords:
mechanical damage of skin, skeletal trauma, blood loss, liver, lipid peroxidation, bile acids, PRP-therapyAbstract
The aim of the work: to establish the role of lipid peroxidation processes in the devepolment of impaired bile production under the influence of skeletal trauma complicated by acute blood loss in combination with mechanical damage of skin, and PRP-therapy effectiveness.
Materials and Methods. The experimental studies were conducted on 186 nonlinear white male rats weighing 180–200 g, which were divided into five groups: the control and four experimental ones. The control group consisted solely of the intact animals exposed to general anesthesia. In the first experimental group, the animals were simulated a mechanical damage of skin, the rats of the second experimental group were subjected to a skeletal trauma complicated by acute blood loss, the third experimental group had both these lesions combined. In the fourth
experimental group, the PRP-therapy was applied using allogeneic platelet-rich plasma injections to the animals with combined trauma. After 3, 7, 14, 21 and 28 days of the posttraumatic period, the common bile duct was catheterized and the bile was collected from the control and experimental groups. The concentration of total bile acids in the bile was subsequently determined in all bile samples. The level of thiobarbituric acid reactive substances (TBARS) was measured in liver homogenate.
Results and Discussion. The conducted studies indicated the enhancement of lipid peroxidation processes in the liver of experimental animals as a result of mechanical damage of skin, skeletal trauma complicated by acute blood loss and the combined trauma model. It was evidenced by the concomitant increase in TBARS values, which did not reach the control level down to the end of experiment. The dynamic of studied indicator was undulant, as the first maximum occurred after 7 days of the posttraumatic period and the second one – after 21 days. The concentration of total bile acids in the bile decreased up to the 7th day, remained at the same level until the 21st day, and, subsequently, increased up to 28th day, though not reaching control values. It was found that a more severe trauma severity led to the greater increase in lipid peroxidation activity as well as more impaired hepatic bile acid synthesis. The administration of the PRP-therapy contributed to a statistically significant decreased TBARS levels in the liver starting from the 21st day of the experiment as well as the increased total bile acids concentration in the bile of rats subjected to combined trauma model as compared to the animals without corrective medication.
The infliction of mechanical damage to skin leads to the enhancement of lipid peroxidation processes in the liver, which results in the obstruction of hepatic bile acid synthesis by endoplasmic reticulum membranes. The intradermal administration of allogeneic platelet-rich plasma injections reduces the systemic manifestations of traumatic disease, which is evidenced by the inhibition of hepatic lipid peroxidation as well as the regression of impaired functionality of endoplasmic reticulum membranes.
References
Zyablitsev, S.V., & Yelsky, V.M. (2020). Syndrom travmatychnoi khvoroby pry cherepno-mozkovii travmi: monohrafiia [Syndrome of traumatic disease in brain injury: monograph]. Kramatorsk: Kashtan [in Ukrainian].
Guryev, S.O., Fil, A.Yu., & Tanasienko, O.M. (2015). Analiz bezposeredikh prychyn smerti u postrazhdalykh iz politravmoiu ta poshkodzhenniam skeleta [Analysis of immediate causes of death in victims with politrauma and skeletal damage]. Travma – Trauma, 16 (4), 7-10 [in Ukrainian].
Hudyma, A.A., Kashchak, T.V., Shepitko, K.V. (2019). Antyoksydantno-prooksydantnyy ta tsytokinovyi balans u piznii period kombinovanoi travmy v eksperymenti [Antioxidant-prooxidant and cytokine balance in the late period of combined trauma in the experiment]. Svit medytsyny ta biolohii – World of Medicine and Biology, 1 (67), 42-47 [in Ukrainian].
Kotelnikov, G.P., Trukhanova, I.G., & Shabanova, A.Yu. (2014). Politravma: sovremennoye sostoyaniye problemy (obzor literatury) [Polytrauma: the current state of the problem (literature review)]. Vrach skoroy pomoshchi – Emergency Doctor, 12, 4-10 [in Russian].
Pfeifer, R., & Pape, H.C. (2016). Diagnostics and treatment strategies for multiple trauma patients. Chirurg., 87 (2), 165-173.
Alves, R., & Grimalt, R. (2016). Clinical indications and treatment protocols with platelet-rich plasma in dermatology. Conde Montero E: PRP in wound healing.Barcelona, 59-72.
Ozdemir, B., & Okte, E.J. (2012). Treatment of intrabony defects with betatricalciumphosphate alone and in combination with plateletrich plasma. Biomed. Mater. Res. B. Appl. Biomater., 100 (4), 976-983.
Levchuk, R.D., Mikhailyuk, I.A., & Merlev, D.I. Sposib modeliuvannia politravmy: pat. na korysnu model 81107 Ukraina, MPK (2006.01) G09V 23/28 № u 2012 13575; zaiavnyk i patentovlasnyk DVNZ “Ternopilskyi derzhavnyi medychnyi universytet imeni I. Ya. Horbachevskoho MOZ Ukrainy”; zayavl. 27.11.12; opubl. 25.06.13, Byul. № 12 [Method of modeling polytrauma: Pat. on utility model 81107 Ukraine, IPC (2006.01) G09В 23/28 № u 2012 13575; applicant and patent owner of I. Horbachevsky Ternopil State Medical University of the Ministry of Health of Ukraine; stated 27.11.12; publ. 25.06.13, Bull. No.12]. [in Ukrainian].
Messora, M.R., Nagata, M.J.H., & Furlaneto, F.A.C. (2011). A standardized research protocol for platelet-rich plasma (PRP) preparation in rats RSBO., 8 (3), 299-304.
Drogovoz, S.M., Gubsky, Y.I., Skakun, M.P., Kovalenko, V.M., Derimedvid, L.V. (2001). Eksperymentalne vyvchennia kholespazmolitychnoi, kholelitiaznoi ta hepatoprotektornoi aktyvnosti novykh likarskykh zasobiv. V: Doklinichni doslidzhennia likarskykh zasobiv: metodychni rekomendatsii; za red. chl.-kor. AMN Ukrayiny O. V. Stefanova [Experimental study of cholespasmolytic, cholelithiasis and hepatoprotective activity of new drugs. In: Preclinical studies of drugs: guidelines; for ed. Corresponding Member Academy of Medical Sciences of Ukraine OV Stefanov]. Kyiv: Avitsenna, 334-351 [in Ukrainian].
Zachepa, O.A., Gudima, A.A., Sushko, Yu.I., & Yaroshenko, T. (2019). Porushennia biokhimichnoho skladu zhovchi za umov poiednanoi travmy zhyvota i hrudnoi klitky v period rannikh proiaviv travmatychnoi khvoroby ta yoho korektsiia tiotryazolinom [Violation of the biochemical composition of bile under conditions of combined trauma of the abdomen and chest in the period of early manifestations of traumatic disease and its correction with thiotriazoline]. Medychna ta klinichna khimiya – Medical and Clinical Chemistry, 21,4 (82), 74-82 [in Ukrainian].
Andreeva, L.I., Kozhemyakin, L.A., & Kishkun, A.A. (1998). Modifikatsiya metoda opredeleniya perekisey lipidov v teste s tiobarbiturovoy kislotoy [Modification of the method for determining lipid peroxides in the test with thiobarbituric acid]. Lab. Delo – Lab. Business, 11, 41-43 [in Russian].
Zachepa, O.A., Gudyma, A.A., & Sushko, Y.I. (2019). Vplyv poiednanoi travmy zhyvota i hrudnoyi klitky na aktyvnist protsesiv lipidnoi peroksydatsii ta antyoksydantnoho zakhystu v period rannikh proiaviv travmatychnoi khvoroby ta yikh korektsiia tiotryazolinom [Influence of combined abdominal and thoracic trauma on the activity of lipid peroxidation and antioxidant protection in the period of early manifestations of traumatic disease and their correction with thiotriazoline]. Aktualni problemy transportnoi medytsyny – Actual Problems of Transport Medicine, 4, 132-143 [in Ukrainian].
Gorban, I.I. (2020). Vplyv hostroi krovovtraty, uskladnenoi ishemiieiu-reperfuziieiu kintsivky, na antyoksydantno-prooksydantnyi balans pechinky ta yoho korektsiia karbatsetamom [Influence of acute blood loss complicated by limb ischemia-reperfusion on antioxidant-prooxidant balance of the liver and its correction by carbacetam]. Zdobutky klinichnoi i eksperymentalnoi medytsyny – Achievements of Clinical and Experimental Medicine, 2, 93-10 [in Ukrainian].
Kashchak, T.V., & Gudyma, A.A. (2018). Intensyvnist protsesiv lipidnoi peroksydatsii ta riven markeriv zapalennia v piznii period kombinovanoi travmy v eksperymenti [Intensity of lipid peroxidation processes and the level of markers of inflammation in the late period of combined trauma in the experiment]. Shpytalna khirurhiia. Zhurnal imeni L. Ya. Kovalchuka – Hospital Surgery. Kovalchuk Journal, 4 (84), 62-68 [in Ukrainian].
Kozak, D.V. (2014). Antyoksydantno-prooksydantnyy balans tkanyny sertsya, lehen i pechinky v dynamitsi politravmy [Antioxidant-prooxidant balance of heart, lung and liver tissue in the dynamics of polytrauma]. Visnyk Vinnytskoho natsionalnoho medychnoho universytetu – Visnyk of Vinnytsia National Medical University, 18 (1), 17-20 [in Ukrainian].
Chekman, I.S., Posokhova, E.A., & Beregovaya, E.G. (1996). Mikrosomal'naya fermentnaya sistema oranizma [Microsomal enzyme system of organisms]. Kyiv [in Russian].
Volotovska, N.V., & Gudyma, A.A. (2011). Rol hemichnoi hipoksii v patohenezi porushen zhovchoutvoriuvalnoi i zhovchovydilnoi funktsii pechinky na tli skeletnoi travmy v rannomu posttravmatychnomu periodi [The role of hemic hypoxia in the pathogenesis of disorders of biliary and biliary function of the liver and the background of skeletal trauma in the early post-traumatic period]. Zdobutky klinichnoi i eksperymentalnoi medytsyny – Achievements of Clinical and Experimental Medicine, 2 (15), 31-33 [in Ukrainian].
Horban, I.I., Hudyma, A. A., Maksymiv, R.V., & Antonyshyn, I.V. (2020). Influence of two-hour tourniquets ischemia of limb and acute blood loss on systemic disorders of the body in the reperfusion period (experimental study). Wiadomości Lekarskie., 7, 1330-1333.
Zachepa, O.A., Gudyma, A.A., & Sushko, Y.I. (2019). Vplyv poiednanoi travmy zhyvota i hrudnoi klitky na aktyvnist protsesiv lipidnoi peroksydatsii ta antyoksydantnoho zakhystu v period rannikh proiaviv travmatychnoi khvoroby ta yikh korektsiia tiotryazolinom [Influence of combined abdominal and thoracic trauma on the activity of lipid peroxidation and antioxidant protection in the period of early manifestations of traumatic disease and their correction with thiotriazoline]. Aktualni problemy transportnoi medytsyny – Actual Problems of Transport Medicine, 4, 132-143 [in Ukrainian].
Zachepa, O.A., Gudyma, A.A., & Sushko, Y.I. (2019). The influence of combined abdominal and thoracic trauma on the bile excretory function of the liver in the period of early manifestations of traumatic disease and their correction with thiotriazoline. J. of Education, Health and Sport., 9 (12), 136-147.
Lacci, K.M. Dardik, A. (2010). Platelet-rich Plasma: support for its use in wound healing. Yale. J. Biol. Med., 83 (1), 1-9.
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