Change of morphological structure of dermal implant from pig skin after “on lay” implantation in experiment
DOI:
https://doi.org/10.11603/2414-4533.2022.1.12918Keywords:
acellular dermal matrix of pigs, onlay implantation, level of vascularization, development of granulation tissueAbstract
The aim of the work: to evaluate the level of vascularization and integration in the experiment, with the development of granulation tissue of the acellular dermal matrix of pigs after implantation on the anterior abdominal wall “on lay” in the early and late period (7, 14, 21 days).
Materials and Methods. The experiment was performed on 6 pigs of the Ukrainian White breed. Under general anesthesia, acellular perforated dermal matrix of pig produced by “Institute of Biomedical Technologies” (Chief Director Prof. Bihuniak V. V.) were implanted on the anterior abdominal wall in the space between the subcutaneous fat and aponeurosis (“on lay”) The quality of engraftment, speed/degree of angiogenesis, condition of granulation tissue, adipose tissue and graft structure were estimated. The postoperative period was uncomplicated, every pig received 1 dose of ceftriaxone 1.0 intramuscularly. From 2 pigs the material was collected on the 7th day, another 2 on the 14th day, and in the last 2 on the 21st day after implantation. Pathomorphological reserches were done in laboratory of I. Horbachevsky TNMU. Sections of the implanted material colored with hematoxylin-eosin were evaluated concerning reactivity of local inflammation, the level of vascularization and replacement by granulation tissue.
Results and Discussion. Studies have shown that acellular dermal matrix after implantation “on lay” causes acute inflammation with slow vascularization in the early period with a moderate increase to 14 and 21 days. The implant acts as a base to support the migration of natural cells and subsequent replacement by granulation tissue as a result of strengthening this area.
Further research is planned concerning integration of biological implants on different levels of the abdominal wall – “sub lay”, preperitoneal.
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