Structural changes of internal organs against the background of simulated acute peritonitis
DOI:
https://doi.org/10.11603/2414-4533.2021.4.12714Keywords:
peritonitis, histochemical examination, Giemsa methodAbstract
The aim of the work: to determine the morphological and histochemical changes of internal organs in experimental animals on the third day of the simulated experimental acute peritonitis.
Materials and Methods. Mature nonlinear female rats weighing 180–220 grams were used in the experiment. Acute widespread peritonitis was modeled by intraperitoneal administration of 30 % fecal suspension in an amount of 1 ml per 100 g of body weight. Animals were withdrawn from the experiment on the third day by decapitation under thiopental anesthesia. Liver, peritoneum, kidney and lung lobes were removed for further pathomorphological and histochemical study. Histological preparations are stained with hematoxylin-eosin. For photo documentation, images from histological specimens were displayed on a computer monitor using a Nicon eclipse C microscope and a Digital Camera SCMOS using ToupWiew software at various magnifications.
Results and Discussion. An increase in blood supply to small vessels without perivascular edema was detected in the lungs. Giemsa and Gram-Weigert bacterial test revealed the presence of single gram-positive bacteria, mainly in the lumen of blood vessels and in the stroma, along with inflammatory infiltrates, as evidenced by color inclusions. Histological examination of the kidneys revealed a slight blood supply to the cortical and cerebral matter. Histological examination of the liver on the third day of experimental peritonitis revealed significant dilation and plethora of central veins, with compression of centrilobular hepatocytes, rather moderate dilation of sinusoids with a small number of erythrocytes and macrophages in their lumens. The reaction of the peritoneum on the third day after the simulation of diffuse peritonitis showed an increase in inflammatory hyperemia, edema, which spread to muscle tissue.
After evaluating the results of our study, it was found that the simulated acute peritonitis was characterized not only by inflammation of the peritoneal leaves, but also by the immune response of other organs.
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