Structural changes of internal organs against the background of simulated acute peritonitis

Authors

  • A. A. Kovalchuk I. Horbachevsky Ternopil National Medical University
  • T. V. Datsko I. Horbachevsky Ternopil National Medical University
  • I. Ya. Dziubanovskyi I. Horbachevsky Ternopil National Medical University
  • V. H. Dzhyvak I. Horbachevsky Ternopil National Medical University

DOI:

https://doi.org/10.11603/2414-4533.2021.4.12714

Keywords:

peritonitis, histochemical examination, Giemsa method

Abstract

The aim of the work: to determine the morphological and histochemical changes of internal organs in experimental animals on the third day of the simulated experimental acute peritonitis.

Materials and Methods. Mature nonlinear female rats weighing 180–220 grams were used in the experiment. Acute widespread peritonitis was modeled by intraperitoneal administration of 30 % fecal suspension in an amount of 1 ml per 100 g of body weight. Animals were withdrawn from the experiment on the third day by decapitation under thiopental anesthesia. Liver, peritoneum, kidney and lung lobes were removed for further pathomorphological and histochemical study. Histological preparations are stained with hematoxylin-eosin. For photo documentation, images from histological specimens were displayed on a computer monitor using a Nicon eclipse C microscope and a Digital Camera SCMOS using ToupWiew software at various magnifications.

Results and Discussion. An increase in blood supply to small vessels without perivascular edema was detected in the lungs. Giemsa and Gram-Weigert bacterial test revealed the presence of single gram-positive bacteria, mainly in the lumen of blood vessels and in the stroma, along with inflammatory infiltrates, as evidenced by color inclusions. Histological examination of the kidneys revealed a slight blood supply to the cortical and cerebral matter. Histological examination of the liver on the third day of experimental peritonitis revealed significant dilation and plethora of central veins, with compression of centrilobular hepatocytes, rather moderate dilation of sinusoids with a small number of erythrocytes and macrophages in their lumens. The reaction of the peritoneum on the third day after the simulation of diffuse peritonitis showed an increase in inflammatory hyperemia, edema, which spread to muscle tissue.

After evaluating the results of our study, it was found that the simulated acute peritonitis was characterized not only by inflammation of the peritoneal leaves, but also by the immune response of other organs.

References

Ross, J. T., Matthay, M. A., & Harris, H. W. (2018). Secondary peritonitis: principles of diagnosis and intervention. BMJ (Clinical research ed.), 361, k1407. Retrieved from: https://doi.org/10.1136/bmj.k1407

Mačiulienė, A., Maleckas, A., Kriščiukaitis, A., Mačiulis, V., Vencius, J., & Macas, A. (2019). Predictors of 30-day in-hospital mortality in patients undergoing urgent abdominal surgery due to acute peritonitis complicated with sepsis. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 25, 6331-6340. Retrieved from: https://doi.org/10.12659/MSM.915435

Park, I., Lee, J.H., Jang, D.H. (2019). Characterization of fecal peritonitis-induced sepsis in a porcine model. The Journal of Surgical Research, 244, 492-501. Retrieved from: https://doi.org/10.1016/j.jss.2019.06.094

Schreyer, A.G. (2018). Inflammatory and infectious abdominal peritoneal and mesenterial processes. Der Radiologe, 58 (1), 10-18. Retrieved from: https://doi.org/10.1007/s00117-017-0334-8

Filippone, A., Cianci, R., Delli Pizzi (2015). CT findings in acute peritonitis: a pattern-based approach. Diagnostic and Interventional Radiology (Ankara, Turkey), 21 (6), 435-440. Retrieved from: https://doi.org/10.5152/dir.2015.15066

Facciorusso, A., Antonino, M., Orsitto, E., Sacco, R. (2019). Primary and secondary prophylaxis of spontaneous bacterial peritonitis: current state of the art. Expert Review of Gastroenterology & Hepatology, 13 (8), 751-759. Retrieved from: https://doi.org/10.1080/17474124.2019.1644167

Volk, S.W. (2015). Peritonitis. Small Animal Critical Care Medicine, 643-648. Retrieved from: https://doi.org/10.1016/B978-1-4557-0306-7.00122-7

Ordoñez, C.A., & Puyana, J.C. (2006). Management of peritonitis in the critically ill patient. The Surgical Clinics of North America, 86 (6), 1323-1349. Retrieved from: https://doi.org/10.1016/j.suc.2006.09.006

Oliveira, L.G., Luengo, J., & Caramori, J.C. (2012). Peritonitis in recent years: clinical findings and predictors of treatment response of 170 episodes at a single Brazilian center. International Urology and Nephrology, 44 (5), 1529-1537. Retrieved from: https://doi.org/10.1007/s11255-011-0107-7

Szeto, C.C. (2014). Peritonitis rates of the past thirty years: from improvement to stagnation. Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis, 34 (2), 151-153. Retrieved from: https://doi.org/10.3747/pdi.2014.00007

Lazarenko, V.A., Lipatov, V.A., Blinkov, Yu.Yu., & Skorikov, D.V. (2008). Eksperimentalnaya model rasprostranennogo kalovogo peritonita [Experimental model of widespread fecal peritonitis]. Chelove i ego zdorovye – Man and His Health, 4, 128-132 [in Russian].

(1986). European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe. Strasbourg.

(2006). Pro zakhyst tvaryn vid zhorstokogo povodzhennia [On protection of animals from cruel treatment]. Law of Ukraine of February 21, 2006 No. 3447-IV. Bulletin of the Verkhovna Rada of Ukraine [in Ukrainian].

Bagriy, M.M., Dibrova, V.A., Popadynets, O.G., & Grishchuk, I.M. (2016). Metodyky morfolohichnykh doslidzhen: monohrafiia [Methods of morphological research: monograph]. Bagriy, M.M., Dibrova, V.A. (Eds.). Vinnytsia: Nova knyha [in Ukrainian].

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Published

2022-02-18

How to Cite

Kovalchuk, A. A., Datsko, T. V., Dziubanovskyi, I. Y., & Dzhyvak, V. H. (2022). Structural changes of internal organs against the background of simulated acute peritonitis. Hospital Surgery. Journal Named by L.Ya. Kovalchuk, (4), 37–43. https://doi.org/10.11603/2414-4533.2021.4.12714

Issue

No. 4 (2021)

Section

EXPERIMENTAL INVESTIGATIONS

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