Dynamics of diuresis and glomerular filtration rate in rats with bilateral Ovariectomy in the period of late manifestations of traumatic disease And efficiency of hormonal replacement therapy

Authors

  • I. I. Lutsiv I. Horbachevsky Ternopil National Medical University
  • D. V. Popovych I. Horbachevsky Ternopil National Medical University
  • O. V. Denefil I. Horbachevsky Ternopil National Medical University

DOI:

https://doi.org/10.11603/2414-4533.2021.2.12298

Keywords:

gonadectomy, cranioskeletal trauma, diuresis, glomerular filtration rate

Abstract

The aim of the work: to determine the effect of cranioskeletal trauma on the dynamics of diuresis and glomerular filtration rate in rats with bilateral ovariectomy in the late manifestations of traumatic disease and to evaluate the effectiveness of hormone replacement therapy.

Materials and Methods. The experiments were performed on 42 nonlinear white female rats weighing 200–220 g. The hypoestrogenic model was performed by surgical removal of the gonads. One month after removal of the gonads in rats, cranioskeletal trauma was simulated. Hormone replacement therapy was performed in a separate subgroup of rats with removed gonads and cranioskeletal trauma. Control groups consisted of intact rats and rats with osteoporosis 1 month after removal of gonads that were not injured. In control groups and after 1 and 2 months of the post-traumatic period in experimental rats, the functional state of the kidneys was determined by water loading and the diuresis and glomerular filtration rate (GFR) were determined.

Results and Discussion. 1 month after removal of the gonads in rats there are violations of the functional state of the kidneys, which are manifested by a decrease in diuresis and a significant slowing of GFR. Applying a cranioskeletal injury after 1 month is an even greater violation of the studied parameters, which prevailed in rats with gonadectomy and did not subside after 2 months of the post-traumatic period. In rats without gonadectomy, diuresis and GFR returned to the control group level 2 months after cranioskeletal injury in response to water load. The use of hormone replacement therapy during the post-traumatic period in rats with gonadectomy caused a significant increase in the studied parameters, compared with rats without correction.

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Published

2021-08-27

How to Cite

Lutsiv, I. I., Popovych, D. V., & Denefil, O. V. (2021). Dynamics of diuresis and glomerular filtration rate in rats with bilateral Ovariectomy in the period of late manifestations of traumatic disease And efficiency of hormonal replacement therapy. Hospital Surgery. Journal Named by L.Ya. Kovalchuk, (2), 46–52. https://doi.org/10.11603/2414-4533.2021.2.12298

Issue

No. 2 (2021)

Section

EXPERIMENTAL INVESTIGATIONS

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