Morphological features of the large intestine in experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes
DOI:
https://doi.org/10.11603/2414-4533.2021.2.12297Keywords:
acute peritonitis, streptozotocin-induced diabetes mellitus, morphological changes of the colonAbstract
The work is a fragment of the research project “Pathogenetic features of the allergic and inflammatory processes course and their pharmacocorrection”, conducted by the Department of Pathological Physiology, Danylo Halytskyi Lviv National Medical University (state registration No. 0116U004503).
The aim of the work: to study the morphological features of the colon in the dynamics of the development of experimental acute peritonitis on the background of streptozotocin-induced diabetes mellitus.
Materials and Methods. The experiment used 48 white male rats. Diabetes mellitus in experimental animals was simulated by a single intraperitoneal injection of streptozotocin (Sigma) at a rate of 60 mg/kg, acute disseminated peritonitis – the introduction of 0.5 ml of 10 % filtered fecal suspension into the abdominal cavity. A morphological study of the colon in animals removed from the experiment on the first, third and seventh days of acute peritonitis on the background of concomitant diabetes mellitus was performed.
Results and Discussion. Morphological examination of the colon of animals on the first day of experimental acute peritonitis in conditions of concomitant streptozotocin-induced diabetes mellitus revealed an increase in the size of the crypts due to stroma edema and lymphohistiocytic infiltration, slight perivascular edema in the subclavian edema. On the third day, thickening of the mucous membrane of the colon, a sharp increase in the depth of the crypts, uneven blood supply to the vessels in the submucosal layer with a predominance of perivascular edema were verified. On the seventh day, a decrease in the thickness of the mucous membrane due to the expansion of the crypts was visualized. Part of the epitheliocytes was in a phase of increased secretory activity, the other part was dystrophically altered, which stimulated increased lymphocytic and histiocytic infiltration.
These changes were accompanied by activation of reactive processes and hyperplasia of lymphoid follicles on the first day of peritonitis on the background of streptozotocin-induced diabetes mellitus, and as the severity of purulent inflammation – hypoplasia of the lymphoid tissue and a decrease in local reactivity(the third and seventh days of the development of acute peritoneal burning in conditions of combined pathology).
References
Mabewa, A., Seni, J., Chalya, P.L., Mshana, S.E., & Gilyoma, J.M. (2015). Etiology, treatment outcome and prognostic factors among patients with secondary peritonitis at Bugando Medical Centre, Mwanza, Tanzania. World Journal of Emergency Surgery, 10, 47. DOI: 10.1186/s13017-015-0042-5
Afuwape, O.O., Ayandipo, O., & Aroso, S. (2020). Base excess and pH as predictors of outcomes in secondary peritonitis in a resource limited setting - A prospective study. Journal of Clinical Sciences, 17 (4), 145-149. DOI: 10.4103/jcls.jcls_18_20
Kim, J.W., Park, J.H., Kim, D.J., Choi, W.H., Cheong, J.C., & Kim, J.Y. (2017). The delta neutrophil indexis a prognostic factor for postoperative mortality in patients with sepsis caused by peritonitis. PLOS One, 12 (8), e0182325. DOI: 10.1371/journal.pone.0182325
Raeeszadeh, M., Hosseini, S.M.J., Khanmohammadi, M.T., Manoochehry, S., & Rasouli, H.R. (2017). Comparison of peritoneal lavage with normal saline and normal saline plus antibiotic in acute peritonitis. Trauma Monthly, 22 (5), e58188. DOI: 10.5812/traumamon.58188
Cercenado, E., Torroba, L., Canto´n, R., Martínez-Martínez, L., Chaves, F., ... & Bouza, E. (2010). Multicenter study evaluating the role of enterococci in secondary bacterial peritonitis. Journal of Clinical Microbiology, 48 (2), 456-459. DOI: 10.1128/JCM.01782-09
Tridente, A., Clarke, G.M., Walden, A., McKechnie, S., Hutton, P., ... & GenOSept Investigators. (2014) Patients with faecal peritonitis admitted to European intensive care units: an epidemiological survey of the GenOSept cohort. Intensive Care Medicine, 40 (2), 202-210. DOI: 10.1007/s00134-013-3158-7
Ross, J.T., Matthay, M.A., & Harris, H.W. (2018). Secondary peritonitis: principles of diagnosis and intervention. British Medical Journal, 361, k1407. DOI:10.1136/bmj.k1407
Steinberg, B.E., Goldenberg, N.M., & Lee, W.L. Do viral infections mimic bacterial sepsis? (2012). The role of microvascular permeability: A review of mechanisms and methods. Antiviral Research, 93 (1), 2-15. DOI: 10.1016/j.antiviral.2011.10.019.
Hernández-Palazón, J., Fuentes-García, D., Burguillos-López, S., Domenech-Asensi, P., Sansano-Sánchez, T.V., & Acosta-Villegas, F. (2013). Analysis of organ failure and mortality in sepsis due to secondary peritonitis. Medicina Intensiva, 37 (7), 461-467. DOI:10.1016/j.medin.2012.07.010
Elías, S.R., Fernández, L.J.O., Choo, U.T., Bordelois, A.M.S., & Lecourtois, M.L. (2019). Factores relacionados con la falla multiorgánica en pacientes con peritonitis. Revista Cubana de Cirugía, 58 (4), e860. Retrieved from: https://www.medigraphic.com/pdfs/cubcir/rcc-2019/rcc194e.pdf
Ramos-Lobo, A.M., Buonfiglio, D.C., & Cipolla-Neto, J. (2015). Streptozotocin-induced diabetes disrupts the body temperature daily rhythm in rats. Diabetology & Metabolic Syndrome, 7, 39. DOI 10.1186/s13098-015-0035-2
Lazarenko, V.A., Lipatov, V.A., Blinkov, Yu.Yu., & Skorikov D.V. (2008). Eksperimentalnaya model rasprostranennogo kalovogo peritonita [Experimental model of diffuse fecal peritonitis]. Kurskiy nauchno-prakticheskiy vestnik “Chelovek i ego zdorovye” – Kursk Scientific and Practical Bulletin “Man and His Health”, 4, 128-132. Retrieved from: https://cyberleninka.ru/article/n/eksperimentalnaya-model-rasprostranennogo-kalovogo-peritonita [in Russian].
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Hospital Surgery. Journal named by L.Ya. Kovalchuk
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish their work in Hospital Surgery. Journal by L. Ya. Kovalchuk agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
