Peculiarities of the enzyme link of antioxidant protection in the early period Of cranioskeletal injury complicated by blood loss in rats with different Hypoxia resistance
DOI:
https://doi.org/10.11603/2414-4533.2021.2.12177Keywords:
cranioskeletal injury, blood loss, resistance to hypoxia, superoxidedismutase, catalaseAbstract
The aim of the work: to elucidate the activity of the enezymic link of the antioxidant defense in the dynamics of the early period of cranioskeletal injury complicated by blood loss in rats with different resistance to hypoxia.
Materials and Methods. The experiments were performed on 148 nonlinear white male rats weighing 180–200 g, which were fed a standard vivarium diet. The rats were preliminarily determined to have individual resistance to hypoxia and two groups were identified: high- and low-resistant (HR, LR). Cranioskeletal trauma was modeled in animals and combined with acute blood loss. After 1, 3 and 7 days, superoxide dismutase (SOD-) and catalase activity was determined in the liver of animals that were not resistant to hypoxia.
Results and its Discussion. High level of SOD- and catalase activity of the liver is a characteristic feature of HR animals in comparison with LR animals of the control group. In response to cranioskeletal trauma, the activity of the studied enzymes in the liver of HR and LR of animals decreased with a maximum after 3 days and remained at the same level for up to 7 days. However, in the HR of animals after cranioskeletal injury, a lesser degree of decrease in the studied parameter was noted. Under the conditions of simulating additional blood loss, violations of the enzyme link of antioxidant defense in the liver were large and increased from 1 to 7 days. However, in the HR of animals, the violation of SOD activity in all periods of the post-traumatic period was less. Catalase activity in response to simulated traumas in the HR of animals after 3 and 7 days of the post-traumatic period was lower both under conditions of simulating only cranioskeletal trauma and its combination with acute blood loss. The results obtained aim at the need to take into account individual resistance to hypoxia in the development of strategies for the diagnosis and correction of traumatic disease in conditions of polytrauma and the differentiated use of substances with antioxidant properties, and requires further in-depth research.
References
Borys, R.M., Goltsev, A.M., & Gozhenko, A.I. (2013). Dynamika pokaznykiv fermentatyvnoi lanky antyoksydantnoho zakhystu v period rannikh ta piznikh proiaviv travmatychnoi khvoroby v umovakh eksperymentalnoi kranio-skeletnoi travmy ta yii korektsii klitynnoiu terapiieiu [The dynamics of fermentative branch of antioxidant protection indices during early and late manifestations of traumatic disease under the conditions of experimental cranio-skeletal injury and its correction by cell therapy]. Arkhiv klinicheskoy i eksperymentalnoy medytsyny – Archives of Clinical and Experimental Medicine, 22 (1), 23-28 [in Ukrainian].
Borys, R.M., Gozhenko, A.I., & Gudyma, A.A. (2013). Dynamika pokaznykiv peroksydnoho okysnennia lipidiv u period rannikh i piznikh proiaviv travmatychnoi khvoroby za umov eksperymentalnoi kranioskeletnoi travmy ta yii korektsii klitynnoiu terapiieiu [The dynamics of lipid peroxidation indices during early and late manifestations of traumatic disease under conditions of experimental cranioskeletal injury and its correction by cell therapy]. Klinichna ta eksperymentalnapatolohiia – Clinical and Experimental Pathology, KHII, 2 (44), 31-34 [in Ukrainian].
Kozak, D.V. (2014). Vplyv karbatsetamu na antyoksydantnyi-prooksydantnyi balans tkanyny sertsia, lehen i pechinky v dynamitsi politravmy [Effect of carbacetam on antioxidant and prooxidant balance of heart lungs and liver tissues in polytrauma dynamic]. Shpytalna khirurhiia – Hospital Surgary, 1 (65), 40-42. DOI: https://doi.org/10.11603/1681-2778.2014.1.4362 [in Ukrainian].
Kozak, D.V. (2012). Osoblyvosti pokaznykiv peroksydnoho okyslennia lipidiv v dynamitsi rannoho i piznoho periodiv politravmy [Dynamics of lipids peroxidation datas in the early and late periods of polytrauma]. Aktualnye problemy transportnoy medytsyny – Actual Problems of Transport Medicine, 3 (29), 103-106 [in Ukrainian].
Korolyuk, M.A., Ivanova, L.I., Mayorova, I.G., & Tokarev, V. Ye. (1988). Metod opredeleniya aktivnosti katalazy [Method for determining the activity of catalase]. Labolatornoye delo – Laboratory Business, 1, 16-19 [in Russian].
Ordynskyi, I.M., & Denefil, O.V. (2019). Zminy peroksydnoho okysnennia lipidiv ta antyoksydantnoho zakhystu v shchuriv riznoi stati, vysoko- i nyzkostiikykh do hostroi hipoksychnoi hipoksii pry immobilizatsiinomu stresi [Changes of lipid peroxidation and antioxidant defense of high and low-resistance to acute hypoxic hypoxia in rats of different sex in immobilizational stress]. Visnyk naukovykh doslidzhen – Bulletin of Scientific Research, 2, 101-104. DOI: https://doi.org/10.11603/2415-8798.2019.2.10270 [in Ukrainian].
Saturska, H.S., & Bondarenko, Yu.I. (2014). Osoblyvosti metabolizmu spoluchnoi tkanyny pry eksperymentalnomu dyfuznomu ishemichno-nekrotychnomu kardiosklerozi u shchuriv iz riznoiu stiikistiu do hipoksii [Features of the connective tissue metabolism in experimental diffuse ischemic necrotic cardiosclerosis in rats w ith different resistance to hypoxia]. Visnyk VNMU – Bulletin VNMU, 18 (2), 425-429 [in Ukrainian].
Sikirynska, D.O., Hudyma, A.A., & Pokhodun, K.A. (2020). Osoblyvosti aktyvatsii protsesiv lipidnoi peroksydatsii v rannii period kranioskeletnoi travmy, uskladnenoi krovovtratoiu, u shchuriv z riznoiu rezystentnistiu do hipoksii [Peculiarities of activation of lipid peroxidation processes in the early period of cranioskeletal injury complicated by blood loss in rats with different resistance to hypoxia]. Medychna ta klinichna khimiia – Medical and Clinical Chemistry, 3, 107-113. DOI: https://doi.org/10.11603/mcch.2410-681X.2020.v.i3.11547 [in Ukrainian].
Tsymbaliuk, G.Yu. (2018). Dynamika zmin v antyoksydantno-prooksydantnii systemi v tkanynakh nyrok pry poiednanii travmi orhaniv cherevnoi porozhnyny na foni hipovolemichnoho shoku ta syndromi ishemii-reperfuzii [Dynamics of changes in antioxidant-prooxidant system in kidney tissues after trauma of abbdominal cavity with hypovolemic shock and ischemia-reperfusion syndrome]. Shpytalna khirurhiia. Zhurnal imeni L.Ya. Kovalchuka – Hospital Surgery. Journal Named by L.Ya. Kovalchuk, 3, 63-69. DOI: https://doi.org/10.11603/2414-4533.2018.3.8898 [in Ukrainian].
Chevari, S., Chaba, I., & Sokey, Y. (1985). Rol superoksiddismutazy v okislitelnykh protsessakh kletki i metod opredeleniya yeye v biologicheskikh materialakh [The role of superoxide dismutase in the oxidative processes of the cell and the method for its determination in biological materials]. Labolatornoye delo – Laboratory Business, 11, 678-681 [in Russian].
Krylyuk, V.O., Grodetskyi, V.K., Kuzminsky, I.V., & Tsymbalyk, G.Yu. (2019). Antioxidant-prooxidant balance in abdominal injury, hypovolemic shock and reperfusion syndrom of the limbs in experiment. The Unity of Science, 1, 149-153.
Horban, I.I., Hudyma, A.A., Maksymiv, R.V., & Antonyshyn, I.V. (2020). Influence of two-hour tourniquets ischemia of limb and acute blood loss on systemic disorders of the body in the reperfusion period (experimental study). Wiad. Lek., LXXIII, 7, 1330-1333.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Hospital Surgery. Journal named by L.Ya. Kovalchuk
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish their work in Hospital Surgery. Journal by L. Ya. Kovalchuk agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
