Morphometric evaluation of structural reconstruction of the venous bed of The large intestine at resections of different volumes of the liver parenchyma

Authors

  • M. S. Hnatjuk I. Horbachevsky Ternopil National Medical University
  • L. V. Tatarchuk I. Horbachevsky Ternopil National Medical University
  • O. M. Procajlo I. Horbachevsky Ternopil National Medical University
  • N. Ja. Monastyrska I. Horbachevsky Ternopil National Medical University

DOI:

https://doi.org/10.11603/2414-4533.2020.4.11779

Keywords:

resection liver, large intestine, venous bed

Abstract

The aim of the work: morphometrically to study the features of structural reoconstruction of the venous bed of the large intestine at resections of different volumes of the liver parenchyma.

Materials and Methods. The studies were performed on 60 male rats, which were divided into 3 groups. Group 1 consisted of 20 intact animals, group 2 – 20 rats after resection of 31.5 % of the liver parenchyma, group 3 – 20 animals after removal of the right and left lateral lobes of the liver (58.1 % of the liver parenchyma). Euthanasia of animals was performed by bloodletting under conditions of thiopental anesthesia 1 month after the start of the experiment. Histological micronutrients were made from the large intestine. The diameters of capillary venules, venules, outer and inner diameters and wall thickness of venous vessels, height of endotheliocytes, their nuclei, nuclear-cytoplasmic relations in endotheliocytes, relative volumes of damaged endotheliocytes were measured. Quantitative indicators were processed statistically.

Results and Discussion. One month after resection of 58.1 % of the liver volume, postresection portal hypertension was detected. It was found that the diameter of the capillary venules increased by 37.0 % and the venules – by 30.7 % (p <0.001). At postresection portal hypertension, the outer diameter of the veins of the large intestine with a pronounced statistically significant difference (p <0.001) increased by 19.4 %, the inner diameter – by 36.6 % (p <0.001), and the wall thickness decreased by 11.9 % (p <0.001). The relative volume of damaged endotheliocytes in the studied experimental conditions increased statistically significantly 22 times (p <0.001) compared with a similar control morphometric parameter. Resection of large volumes of liver parenchyma leads to postresection portal hypertension and significant remodeling of the venous bed of the colon, which is characterized by pronounced dilation and plethora of venous vessels, violation of their venous drainage function, hypoхia, dystrophic, necrobiotic, infiltrative and sclerotic processes in the studied organ.

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Published

2021-02-15

How to Cite

Hnatjuk, M. S., Tatarchuk, L. V., Procajlo, O. M., & Monastyrska, N. J. (2021). Morphometric evaluation of structural reconstruction of the venous bed of The large intestine at resections of different volumes of the liver parenchyma. Hospital Surgery. Journal Named by L.Ya. Kovalchuk, (4), 24–28. https://doi.org/10.11603/2414-4533.2020.4.11779

Issue

No. 4 (2020)

Section

EXPERIMENTAL INVESTIGATIONS

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