Efficacy of platelet-rich blood plasma in induction of muscle tissue healing in An experimental study

Authors

  • V. H. Dzhyvak I. Horbachevsky Ternopil National Medical University
  • I. M. Klishch I. Horbachevsky Ternopil National Medical University

DOI:

https://doi.org/10.11603/2414-4533.2020.3.11461

Keywords:

muscle injury, platelet-rich blood plasma, PRP, lipid peroxidation, antioxidant system

Abstract

The aim of the work: to evaluate the effectiveness of platelet-rich blood plasma in the induction of muscle healing in an experimental study.

Materials and Methods. The experiment was performed on 92 laboratory rats, which were on the standard diet of the vivarium. Experimental animals were divided into three groups: control – 12 intact, the first experimental group – 40 with traumatic thigh muscle injury without the introduction of platelet-rich blood plasma, the second experimental group – 40 with traumatic thigh muscle injury and the introduction of platelet-rich blood plasma. Preparation of PRP took place in two stages. The effect of platelet-rich blood plasma correction on changes in lipid peroxidation (LPO) and antioxidant protection (AOC) and morphological changes in muscle tissue was analyzed in an experiment in traumatic muscle injury with platelet-rich blood plasma. Biological material was collected on the 1st, 7th, 14th, 21th day after traumatic muscle injury.

Results and Discussion It was found that the use of platelet-rich blood plasma reduces the activity of lipid peroxidation, which reduces the signs of inflammation in the injured muscle, which are morphologically manifested on the 7th, 14th day by the following changes: on the 7th day the area of ​​damage was represented by moderate dystrophic changes in muscle fibers, slight edema and an increase in the number of fibroblasts. After 14 days, a small amount of collagen stroma with formed granulation tissue, residual edema and isolated dystrophic muscle changes were visualized, necrotic manifestations were not observed. On day 21, loose collagen fibers were replaced with a hyaline stroma at the site of injury. The number of lympho- and plasma cells, fibroblasts, mast cells decreased sharply and perivascular edema practically disappeared. The vessels of the microcirculatory tract were clearly visualized. The structure of the muscle fibers themselves improved: the sarcoplasm received dyes more intensively, the nuclei regained their localization.

References

Dzhyvak, V., Klishch, I., Datsko, T., & Khlibovska, O. (2020). Influence of PRP on morphological changes in muscle in the early period after traumatic muscle injury in the experiment. Journal of Education, Health and Sport, 10 (6), 171-178. eISSN 2391-8306. DOI: http://dx.doi.org/10.12775/JEHS.2020.10.06.019. DOI: https://doi.org/10.12775/JEHS.2020.10.06.019

Blessing,T.O., Erin, E.F., Scerbo, М., Tomasek, J.S., Wade, C.E., & Holcomb, J.B. (2017). Trends in 1029 trauma deaths at a level 1 trauma center. Injury, 48 (1), 5-12.

Nikanorov, O.K. (2015). Problema travmatyzmu v ihrovykh vydakh sportu ta perspektyvy vykorystannia zasobiv fizychnoi reabilitatsii [The problem of injuries in game sports and prospects for the use of physical rehabilitation]. Sportyvna medytsyna – Sports Medicine, 1-2, 82-86 [in Ukrainian].

Hallen, A., & Ekstrand J. (2014). Return to play following muscle injuries in professional footballers. J. Sports Sc., 32 (13), 1229-1236. DOI: https://doi.org/10.1080/02640414.2014.905695

Magdalena Kozakowska, Katarzyna Pietraszek-Gremplewicz, Alicja Jozkowicz, & Jozef Dulak (2015). The role of oxidative stress in skeletal muscle injury and regeneration: focus on antioxidant enzymes. J Muscle Res. Cell Motil., 36, 377-393. DOI 10.1007/s10974-015-9438-9. DOI: https://doi.org/10.1007/s10974-015-9438-9

Rodrigo Pereira Martins, Diane Duarte Hartmann, Jefferson Potiguara de Moraes, Felix Alexandre Antunes Soares & Gustavo Orione Puntel (2016). Platelet-rich plasma reduces the oxidative damage determined by a skeletal muscle contusion in rats. Platelets, 27

Walter Geremicca, Carla Fonte, & Sisto Vecchio (2010). Blood components for topical use in tissue regeneration: evaluation of corneal lesions treated with platelet lysate and considerations on repair mechanisms. Blood Transfus, 8 (2), 107-112.

Ozlem Karabudak Abuaf, Hamza Yildiz, Hüseyin Baloglu, Memet Ersan Bilgili, Hasan Aktug Simsek, & Bilal Dogan (2016). Histologic evidence of new collagen formulation using platelet rich plasma in skin rejuvenation: A Prospective Controlled Clinical Study. Ann. Dermatol., 28 (6), 718-724. Retrieved from: https://doi.org/10.5021/ad.2016.28.6.718. DOI: https://doi.org/10.5021/ad.2016.28.6.718

Cho, J.W., Kim, S.A,& Lee, K.S. (2012).Platelet-rich plasma induces increased expression of G1 cell cycle regulators, type I collagen, and matrix metalloproteinase-1 in human skin fibroblasts. Int. J. Mol. Med., 29, 32-36.

Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. Official Journal of the European Union, 2010, L276, 33-79.

(2011). Guide for the care and use of laboratory animals. 8th ed. Washington: The National Academies Press.

Stalnaya, I. D. & Garishvili, T.G. (1997). Metod opredeleniya malonovogo dialdegida s pomoshchyu tiobarbiturovoy kisloty [Method of determination of malonic dialdehyde with the help of thiobarbituric acid]. Mosсow: Meditsina [in Russian].

Korolyuk, M.A., Ivanova, L.I., Mayorova, I.G., & Tokarev, V.E. (1988). Metod opredeleniya aktivnosti katalazy [Method of determining catalase activity]. Laboratornoe delo – Laboratory Work, 1, 16-19 [in Russian].

Levitsky, A.P., Pochtar, V.M., Makarenko, O.A., & Gridina L.I. (2006). Antyoksydantno-prooksydantnyi indeks syrovatky krovi shchuriv z eksperymentalnym stomatytom i yoho korektsiia zubnymy eliksyramy [Antioxidant-prooxidant index of serum of rats with experimental stomatitis and its correction with dental elixirs]. Odeskyi medychnyi zhurnal – Odesa Medical Journal, 1, 22-25 [in Ukrainian].

Stalnaya, I.D. (1977). Metod opredeleniya dienovyih konyugat vysshih zhirnykh kislot. [Method for determining diene conjugates of higher fatty acids] Orekhovich, V.N. (Ed.). Sovremennye metody v biokhymii – Modern Method in Biochemistry. Mosсow: Meditsina [in Russian].

Bagriy, M., Dibrova ,V., Popadinets, O., & Grishchuk, I. (2016). Metodyky morfolohichnykh doslidzhen [Methods of morphological researches]. Vinnytsia: Nova Knyha [in Ukrainian].

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Published

2020-10-23

How to Cite

Dzhyvak, V. H., & Klishch, I. M. (2020). Efficacy of platelet-rich blood plasma in induction of muscle tissue healing in An experimental study. Hospital Surgery. Journal Named by L.Ya. Kovalchuk, (3), 36–43. https://doi.org/10.11603/2414-4533.2020.3.11461

Issue

No. 3 (2020)

Section

EXPERIMENTAL INVESTIGATIONS

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