Morphometric assessment of structural changes of arterial and venous Endotheliocytes and veins of the testes in the conditions of postresection Arterial hypertension

Abstract

The aim of the work: to study morphometrically the features of structural rearrangement of endothelial cells of arteries and veins of the testes at postresection arterial pulmonary hypertension.

Materials and Methods. Morphologically, the structural changes of endothelial cells of the arteries and veins of the testes of 50 rats, which were divided into 3 groups, were studied. Group 1 included 15 intact animals, group 2 – 25 rats with postresection arterial hypertension in the small circulation and compensated cor pulmonale, group 3 – 10 experimental animals with pulmonary arterial hypertension and decompensated cor pulmonale. One month after the start of the experiment, rats were euthanized by bloodletting under thiopental anesthesia. The height of endothelial cells of arteries and veins, the diameter of their nuclei, nuclear-cytoplasmic relations in endotheliocytes, and the relative volume of damaged cells were measured in the testes. Quantitative indicators were processed statistically.

Results and Discussion. It was established that right pulmonectomy leads to pulmonary hypertension, cor pulmonale, venous plethora in the organs of the great circle of blood circulation and structural changes in the testes. The most pronounced changes were found in the testes at postresection pulmonary hypertension and decompensated cor pulmonale. Nuclear-cytoplasmic ratios in the endothelial cells of the arteries of the left testis increased by 7.6 %, the right – by 4.7 % (p <0.05), the relative volumes of damaged endothelial cells increased by 11.7 and 9, respectively, 3 times (p <0.001), in venous endotheliocytes changed by 6.6 %, 5.8 % (p <0.001), 20.8 and 17.5 times, respectively (p <0.001). Thus, the combination of postresection arterial pulmonary hypertension with cor pulmonale decompensation leads to disorders of cellular structural homeostasis, growth of relative volumes of damaged endotheliocytes in arte­ries and veins of testes, atrophy, dystrophy, tissue infiltrations and necrobiosis. The detected processes dominate in the venous bed аnd structures of the left testis.