Experimental reasoning of interpheron 2b intraperitoneal application for treatment of acute peritonitis

Abstract

The aim of the work: to investigate experimentally the feasibility of intraperitoneal application of interferon α2b for the treatment of acute peritonitis.

Materials and Methods. 64 white nonlinear rats were used in the experiment. Peritonitis was modeled by intraperitoneal puncture application of 20 % autofeces solution. In 12 h after the simulation  laparotomy and sanation of the abdominal cavity was performed. In the control group (32 animals), peritoneal cavity was sanated with decamethoxin solution and 0.9 % NaCl solution. In the experimental group (32 animals) thereafter, a solution of interferon α2b at a dose of 0.3 million IU per 100 g of mass was applied into the abdominal cavity. In 6, 12, 24 and 48 h, a relaparotomy was performed and the parietal peritoneum was removed for histological examination. The tissues were stained with hematoxylin-eosin.

Results and Discussion. In the control group in 6 h after sanation on the background of distinct inflammatory changes of the parietal peritoneum, there are signs of an improper reaction of a nonspecific cellular level of protection, the manifestations of which are activated only after 12 h; the initiation of a specific link occurs after 24 h, and within 48 h no signs of regeneration processes are detected. In the study group in 6 h there are signs of an adequate response of the nonspecific cellular protection against the background of less pronounced inflammatory changes of the parietal peritoneum; after 12 h signs of activation of a specific link, and after 48 h – signs of the development of regeneration processes.