Changes of antioxidant system in experimental animals after combined Abdomino-skeletal trauma and ischemia reperfusion of the lower extremities

Authors

  • S. V. Garian Ukrainian Scientific and Practical Center of Emergency and Disaster Medicine, Ministry of Health of Ukraine, Kyiv

DOI:

https://doi.org/10.11603/2414-4533.2019.4.10709

Keywords:

ischemia, reperfusion, tourniquet, experiment, combined trauma, catalase, bleeding

Abstract

The aim of the work: to study the effect of limb reperfusion in a model of combined abdomino-skeletal injury on changes in antioxidant protection in experimental animals.

Materials and Methods. The experiment used 130 adult white male Wistar rats weighing 190–220 g, which were on a standard diet of vivarium.

All animals were divided into groups: control (CG) and three experimental (EG): control group – intact animals (10 animals); first experimental group (EG-1) – simulated hip fracture, massive external bleeding and lower limb ischemia-reperfusion (40 animals); second experimental group (EG-2) – modeled hip fracture, massive external blood loss and closed trauma of abdominal organs (40 animals); the third experimental group (EG-3) – modeled closed abdominal trauma, skeletal trauma, massive external blood loss, and lower extremity ischemia-reperfusion (40 animals).

The animals of the experimental groups were removed from the experiment under conditions of thiopental sodium anesthesia by total blood flow from the heart 3, 7, 14 and 21 days after trauma modeling. Animal blood was collected for the study.

The activity of catalase was determined in the serum, and the antioxidant-prooxidant index was calculated based on previously obtained data on the content of reagents to thiobarbituric acid (TBA-active products).

Results and Discussion. The results suggest that limb reperfusion causes the development of prolonged oxidative stress in response to increasing catalase levels. The most pronounced changes were observed in the group of experimental animals, where ischemia of lower extremity reperfusion was present along with the combined abdominal-skeletal injury. It was it that caused the development of long-lasting systemic changes and, accordingly, an imbalance of adaptation-compensatory mechanisms aimed at eliminating pathological factors.

The combination of abdominal-skeletal trauma and ischemia of lower reperfusion causes prolonged oxidative stress and depletion of antioxidant systems, as evidenced by a decrease in the level of API, which after 21 days was statistically less than the control values ​​(p <0.05) by 13.3 %.

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Published

2020-01-10

How to Cite

Garian, S. V. (2020). Changes of antioxidant system in experimental animals after combined Abdomino-skeletal trauma and ischemia reperfusion of the lower extremities. Hospital Surgery. Journal Named by L.Ya. Kovalchuk, (4), 42–49. https://doi.org/10.11603/2414-4533.2019.4.10709

Issue

No. 4 (2019)

Section

EXPERIMENTAL INVESTIGATIONS

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