DETERMINATION OF ANTIMICROBIAL ACTIVITY OF SAXIFRAGE PIMPINELLA DRY EXTRACT

Abstract

The aim of the work. Determination of antimicrobial activity of subterraneous organs of the saxifrage pimpinella dry extract.

Materials and Methods.  The object for research was chosen subterraneous organs of the saxifrage pimpinella dry extract (pimpenella thick extract-1, obtained by extraction with 75 % ethanol and pimpenella thick extract-2, extractant 85 % ethanol). Five archival strains were used as test cultures: gram-positive microorganisms Staphylococcus aureus ATCC 6538, spore culture Bacillus subtilis ATCC 6633, gram-negative culture of Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 9027. Antifungal effect was found on Candida albicans ATCC 885-653.

The bacteriostatic properties of the subjects under investigation were determined by the growth of reference strains of microorganisms in the native aqueous solution of the saxifrage pimpinella and in dilution 1:2 and 1:4 in the meat-peptone broth; bactericidal – in the absence of growth of the content of samples with dilution of the extract on dense live nutrients (meat-peptone agar – MPA) for gram-positive microorganisms S. aureus, B. subtilis, for the gram-negative culture of E. coli, P. aeruginosa. Agar Saburo was used for yeast-like fungi of the Candida (C. albicans) genus.

Results and Discussion. The subterraneous organs of the saxifrage pimpinella dry extract has a wide range of antibacterial activity. The native extract of pimpenella thick extract-1 in relation to the test strains of S. aureus, B. subtilis, C. albicans showed a bactericidal effect. It manifested a bacteriostatic effect to P. aeruginosa, but in relation to E. coli, the antimicrobial effect was not detected. Extract in dilution 1:2 and 1:4 showed bactericidal action against B. subtilis, bacteriostatic – to S. aureus, C. albicans and no antimicrobial activity against E. coli and P. aeruginosa.

The native sample of pimpenella thick extract-2 in relation to all 5 tested strains tested showed bactericidal activity; in dilution of 1:2 and 1:4 showed bactericidal and bacteriostatic effects on S. aureus, B. subtilis, C. albicans. Antimicrobial activity against E. coli and P. aeruginosa was not detected.

The antimicrobial activity of the native saxifrage pimpinella dry extract was also studied by in vitro method of diffusion into agar – the method of "wells". Strains S. aureus, C. albicans, B. subtilis, P. aeruginosa, E. coli showed sensitivity to the native subterraneous organs of the saxifrage pimpinella dry extract. Gram-positive bacterial strains of S. aureus, as well as C. albicans, B. subtilis fungi, are most susceptible to pimpenella thick extract-2, and pimpenella thick extract-1 manifested relatively less sensitivity. Gram-negative cultures P. aeruginosa and E. coli also showed moderate sensitivity to pimpenella thick extract-1, but significantly greater sensitivity to pimpenella thick extract-2.

Conclusions. 1. It was experimentally established that both investigated samples of the subterraneous organs of the saxifrage pimpinella dry extract manifest antibacterial activity.

2. The subterraneous organs of the saxifrage pimpinella dry extract showed a more pronounced antimicrobial activity in relation to the gram-positive microflora, therefore it is promising for the creation of a medicinal product with antimicrobial properties.