INTENSITY OF OXIDATIVE PROCESSES IN A RAT'S ORGANISM, DAMAGED BY TETRACHLOROMETHANE AFTER USING LANCIFOLIA HOSTA LEAVES TINCTURE
DOI:
https://doi.org/10.11603/2312-0967.2016.3.6823Keywords:
hosta lancifolia leaves tincture, tetrachloromethane liver injury, oxidative processes, antioxidate systemAbstract
Introduction. Lipid peroxidation activation (LPA) is nowadays considered as one of the main pathogenic mechanism of liver injury [3].
That is why lately so much attention is paid to the problem of search and creation of medicinal products with antioxidant properties, especially, substances of natural origin.
Our attention has been attracted by a widely cultivated plant, which is used in folk medicine and is called hosta lancifolia. The tincture, based on this plant, is made and standardized at the department of National University of Pharmacy.
The aim of our study was to examine the effect of hosta lancifolia tincture on oxidative processes in the rat’s organism, damaged by tetrachloromethane.
Materials and methods. The experiments were conducted on white outbred male rats weighing 170-180 g. In order to investigate the efficacy of tincture as antioxidant agent we used the model of animals’ liver injury (CCl4). The object of the study was 50 º hosta lancifolia leaves tincture at a dose of 0.15 ml/kg of body weight. As a comparator, we have chosen a phytogenic hepatoprotector silymarin (manufacturer - JSC "Sofarma). The experimental animals were divided into four groups (18 animals per group): 1 – control; 2 – rats, damaged by tetrachloromethane; 3 – rats, damaged by tetrachloromethane and silymarin correction; 4 – rats, damaged by tetrachloromethane and hosta lancifolia tincture correctiom.
Euthanasia of rats was performed under thiopental anesthesia on the 4th, 7th and 14th day of the experiment.
For the investigation there were used blood serum and liver of experimental rats. The activity of oxidative processes and antioxidant system after the injection of corrective factors were evaluated by the content of TBA-active products (TBA-AP) [12], ceruloplasmin (CP) [5], reduced glutathione (RG) [13], the activity of catalase (CT) [6] and content of 2,4-dynitrofenilhidrazoniv (2,4 DNFH) [2].
Results and discussions. The maximum increase of intermediate products of free radical oxidation in blood serum was observed on the 7th day of injury (3.7 times), and in the liver it was observed on the 14th - (2.2 times). After the injection of tincture and comparator into the damaged body, there was observed reduction of TBA-AP.
The research of OMP indicators showed that the content of 2,4-DNFH of neutral (370 nm) and main character (430 nm).The content of this indicator decreased significantly after using the selected drugs.
As a result of radical oxidation processes, the antioxidant system in the organism of experimental animals was weakened, as evidenced by the decrease of catalase activity and content of reduced glutathione in the blood serum of rats after the injury. Due to the indicators, there was increase of catalase activity and reduced glutathione content during three periods of experiment after hosta tincture and phytogenic hepatoprotector correction.
After the injury of rats’ liver there was observed the increase of ceruloplasmin content in the blood serum during the whole experiment. According to results the effect of silymarin on the CP content in the blood serum on the 4th and 14th days was bigger, than of tincture. On the 7th day of the experiment, the efficiency of the tincture turned out to be on the same level with comparator.
Conclusion. Result of investigation: a positive effect of hosta lancifolia leaves tincture on the processes of free radical oxidation (decrease in the content of TBA-active products and products of proteins oxidative modification), activated after damaging the rats by tetrachloromethane. Activity of catalase, glutathione content and reduction of ceruloplasmin in the affected body is increased at application of tincture at a dose of 0.15 ml/kg, and indicated on its positive effect on indexs of antioxidant system.
This suggests about the prospects of further investigation of the pharmacologic properties of hosta lancifolia leaves tincture.
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