ДОСЛІДЖЕННЯ ФЕНОЛЬНИХ СПОЛУК РОЗХІДНИКА ЗВИЧАЙНОГО (GLECHOMA HEDERACEA L.)
DOI:
https://doi.org/10.11603/2312-0967.2015.3.4948Abstract
THE INVESTIGATION OF COMPOUNDS PHENOL ORIGIN GROUND IVY (GLECHOMA HEDERACEA L.)
M. S. Garnyk
NationalPyrogovMemorialMedicalUniversity, Vinnytsia
Summary: compounds of phenol origin have been quantified in ground ivy herb, they are hydroxycinnamic acids (4.20-6.47 %), flavonoids (0.77-1.35 %), oxidized phenols (7.75-8.51 %). Flavonoid groups (rutin, hyperozide, apigenin, luteolin, isoquercitrin), coumarines (scopoletin, umbelliferone) hydroxycinnamic acids (rosmarinic, chlorogenic, caffeic, p-coumaric) have been identified by the method of HPLC and their content has been identified.
Key words: ground ivy, compounds of phenol origin, spectrophotometry, permanganatometry, high performance liquid chromatography.
Introduction. The ability to synthesize and accumulate huge amount of phenolic compounds is a characteristic feature of the representatives of the plant world. Natural phenols exhibit high biological activity. They take part in various physiological processes: respiration, photosynthesis, growth, development and reproduction of plants; some polyphenols protect plants from pathogens and fungal diseases.
Medicinal products based on phenolic compounds are widely used as anti-microbial, anti-inflammatory, hemostatic, choleretic, diuretic, hypotensive, tonic, astringent and purgative means. Most of the phenolic compounds of plant origin have low toxicity and do not exhibit side effects.
Previous studies found that grass of ground ivy contains a number of phenolic compounds namely: hydroxycinnamic acids, flavonoids, tannins. Therefore the purpose of the study was to determine the quantitative content of phenolic compounds by spectrophotometric method and individual substances by high performance liquid chromatography.
Methods. The determination of phenolic compounds recalculated to gallic acid has been performed by spectrophotometric method at a wavelength of 270 nm, the amount of hydroxycinnamic acids recalculated to chlorogenic acid – at a wavelength of 327 nm, the amount of flavonoids recalculated to rutin – at a wavelength of 410 nm; total oxidized amount of phenols has been determined by permanganatometrical titration. To separate the total amount of phenolic compounds into individual components high performance liquid chromatography was used.
Results and discussion. The content of phenolic compounds in grass of ground ivy was 3.15-3.41% in the sample number 1 (raw materials harvested in Ternopil region) and 2.98-3.29 % in the sample number 2 (materials harvested in Vinnytsia region), the total content of oxidized phenols is 8.34-8.51 % and 7.75-8.14 %, the amount of flavonoids – 0.87-1.35% and 0.77-1.04 %, the amount hydroxycinnamic acids – 4.20-6.47 % and 4.84-6.36 % respectively for the objects number 1 and number 2.
Individual quantitative content of phenolic compounds in the grass of ground ivy was identified by the method of HPLC. Flavonoids (rutin, hyperozide, apigenin, luteolin, isoquercitrin) were identified in the studied objects, as well as coumarins (skopoletyn, umbelliferone), hydroxycinnamic acid (rosmary, chlorogenic, coffeic, p-coumaric), and their quantitative content was determined.
Among flavonoid compounds of ground ivy the greatest number falls to rutin – 0.40 and 0.23 % respectively in objects number 1 and 2. As for hydroxycinnamic acid the highest content of rosmarinic acid is observed in the sample number 2 – 1.13 %. The content of chlorogenic acid was also significant and made 0.84 % (№ 1) and 0.81 % (№ 2) in studied objects.
Conclusions. 1. Quantitative content of phenolic compounds: total amount of oxidative phenols, total amount of hydroxycinnamic acids, total amount of flavonoids were detected in the studied samples of ground ivy.
2. Quantitative content of flavonoids (rutin – 0.23-0.40 %, hyperozide – 0.17-0.34 %, luteolin – 0.05-0.1% isoquercіtrіn – 0.02-0.03 %, apigenin – 0.06 %), hydroxycinnamic acids (chlorogenic – 0.81-0.84 %, rosmarinic – 0.2-1.13%, coffeic – 0.06 %, p-coumaric – traces) and coumarins (skopoletyn – 0.11-0.12 %, umbelliferonе – 0.02 %) was identified and defined by the method of HPLC.
Downloads
Published
How to Cite
Issue
Section
License
The authors who publish their materials in this journal agree with the following terms:
- Authors reserve the right to authorship of their work and assign to the journal the right to first publish this work under the terms of the Creative Commons Attribution License, which allows other persons to freely distribute the published work with a mandatory reference to the authors of original work and the first publication of work in this journal
. - Authors have the right to make independent extra-exclusive work agreements in the form they are published by this journal (for example, posting work in an electronic repository of an institution or publishing as part of a monograph), provided that the link to the first publication of the work in this journal is maintained.
Journal policy allows and encourages publication of manuscripts on the Internet (for example, in institutions repositories or on personal websites), both before the publication of this manuscript and during its editorial review, as it contributes to productive scientific discussion and positively affects the efficiency and dynamics of the citation of the published work (see The Effect of Open Access).
