ВПЛИВ ІНФУЗІЙНИХ РОЗЧИНІВ ЛАКТОПРОТЕЇНУ З СОРБІТОЛОМ ТА HAESLX- 5% НА БІОХІМІЧНІ МАРКЕРИ ФІБРОГЕНЕЗУ ЛЕГЕНЕВОЇ ТКАНИНИ В ЩУРІВ З ОПІКОВОЮ ТРАВМОЮ ШКІРИ

Abstract

INFLUENCE of laktoproteinum with sorbitol and HAES-LX-5% infusion’S solutions on biochemical markers of fibrogenesis lung tissue in rats with skin burn trauma

Ocheretnyuk A.А.       

VinnytsiaNationalMedicalUniversityby M.I. Pyrogov

Summary. It is shown that in a case of burn disease, especially on day 3, remodeling of connective tissue and oxidative damage phospholipid lungs takes place. Infusion of colloidal hyperosmolar solution - HAES-LX- 5% and lactoproteinum solution with sorbitol equally causes antifibrogenic effect and reduces the severity of oxidative degradation of membrane phospholipids in rats with skin burns. Their protective effect is the most expressive on day 7 of the experiment.

Keywords: infusion therapy, fibrogenesis, phospholipids, HAES-LX-5%, lactoproteinum with sorbitol.

Introduction.

Trigger mechanisms for alteration of lung tissue under conditions of skin burn injuries is considered a development of oxidative stress accompanied by accumulation of oxidative modification of lipids and destruction of cell membranes. This process is characterized also by remodeling of connective tissue and activation of fibrogenesis processes in the lung tissues. It is quite clear that the efficacy of potential correctors of the lung functional condition is largely determined by their ability to contain the accumulation of reactive free radicals, reduce lesion of bi-lipidic layer of cell membranes, and to detect depressing effect on fibrogenesis in the lung tissue.

Methods.

The contents of biochemical studies: serum samples were tested for markers of degradation of connective tissue and fibrogenesis (free oxyproline, transforming growth factor-β1), lung homogenate was tested for contents of phosphatidylcholine and lysophosphatidylcholine.

Results and discussion.

Skin burn injury causes diverse biochemical and pathophysiological changes in rats in whole and particularly in the lung tissue. Our research has shown that burn disease is accompanied by distortion of phospholipid spectrum of lung tissue, which manifested by decrease of phosphatidylcholine content and increase of its oxidation product - lysophosphatidylcholine. Covalent modification of phospholipid membranes in case of burn disease leads to disruption of the permeability of cell membranes and disorders in the cellular pumps required to transport materials and create ion concentration gradient on both sides of cell membrane. Along with these changes, the burn disease is characterized by connective tissue remodeling and fibrogenesis processes activation, as convincingly proved by growth in serum free oxyproline and transforming growth factor TGF-β1. Pharmacological correction of burn disease using HAES-LX-5% and lactoproteinum with sorbitol solutions somewhat normalized ratio of phosphatidylcholine/lysophosphatidylcholine in cell membranes of the lung tissue and prevented remodeling of connective tissue and fibrogenesis activation. HAES -LX-5% was proven at least not inferior versus a reference medicine - lactoproteinum with sorbitol-  in terms of the above-said effects.

Conclusions.

Skin burn injury in rats accompanied by the development of lung imbalance of lung phospholipids (phosphatidylcholine content reduced by 35-47% and the lysophosphatidylcholine grew by 34-66%) and increasing content of connective tissue destruction (free oxyproline - by 60-97%) and fibrogenesis (TGF-β1 – by 58-137%) markers in serum.

Administration of colloidal-osmotic solution HAES-LX-5% and lactoproteinum with sorbitol suppressed development of connective tissue remodeling processes of fibrogenesis and oxidative lesion of lungs phosphorlipidic bilayer.