STUDY OF THE EFFECT OF S-HETEROCYCLIC SUCCINATES ON MICROCLONAL PROPAGATION OF ORNAMENTAL PLANTS

Authors

DOI:

https://doi.org/10.11603/2312-0967.2024.3.14838

Keywords:

S-heterylsuccinates, growth regulators, microclonal propagation, synthesis, toxicity, rhizogenesis

Abstract

The aim of the work. Investigation of the impact of new derivatives of S-hetaryl succinates on the microclonal propagation of ornamental plants: clone 112 of Paulownia tomentosa (Thunb.) Steud. and Rosa damascena Mill. 'Lada'; determination of lipophilicity as one of the important factors influencing the manifestation of biological activity.

Materials and Methods. For the synthesized compounds, in silico toxicity screening was performed using the online services TEST and GUSAR, and the functional state of male spermatozoa was experimentally studied in vitro. For performing specific stages of calculating the physicochemical characteristics of the compounds, ACD-I-Labs was used. To evaluate the biological effect of the compounds on rhizogenesis, microclonal propagation technology on Murashige-Skoog nutrient medium was employed. The studied compounds were added before the sterilization of the nutrient medium. The control consisted of nutrient medium without growth regulators.

Results and Discussion. S-hetaryl succinates, which were previously synthesized via a nucleophilic substitution reaction based on 4-chloroquinolines and 4-chloropyridine and the residue of mercaptosuccinic acid (the structure of the synthesized compounds was confirmed by 1H, 13C NMR spectra), demonstrated a low toxic profile and high growth-stimulating activity in the microclonal propagation of clone 112 of Paulownia tomentosa (Thunb.) Steud. and Rosa damascena Mill. 'Lada'. The studied compounds do not require complex chemical synthesis, are minimally toxic, and stimulate rhizogenesis at low concentrations – 10⁻⁵ to 10⁻⁷ mol/L, making them environmentally and economically favorable candidates in the group of growth regulators. The high growth-regulating activity allows them to be recommended for further in-depth study in other plant propagation and cultivation technologies.

Conclusions. The addition of 2-((7-chloroquinolin-4-yl)thio)succinic acid (QS-3) to the nutrient medium reliably increased the number and length of roots in plants, with a maximum rhizogenesis frequency of 92 % in Paulownia tomentosa and 84 % in Rosa damascena, which is 16 % and 12 % higher than in the comparison reagent, respectively. The obtained results allow this compound to be recommended for practical use and can serve as a platform for further structural optimization in the development of modern growth regulators for vegetative plant propagation.

Author Biographies

M. P. Zavhorodnii, Municipal Institution of Higher Education "Khortytsia National Educational and Rehabilitation Academy" of the Zaporizhzhia Regional Council

PhD (Biology), Associate Professor of the Landscape Gardening Department

N. P. Derevianko, Municipal Institution of Higher Education "Khortytsia National Educational Rehabilitation Academy" of the Zaporizhzhia Regional Council

PhD (Agricultural Sciences), Associate Professor of the Landscape Gardening Department

Т. E. Shkopynska, Medical Professional College Zaporizhzhia State Medical University

PhD (Agricultural Sciences), Head of the Pharmacy Department, Lecturer of the highest qualification category

O. A. Brazhko, Zaporizhzhia National University

DSc (Biology), Professor of the Chemistry Department

M. M. Kornet, Zaporizhzhia National University

PhD (Biology), Associate Professor of the Chemistry Department

V. I. Gencheva, Zaporizhzhia National University

PhD (Biology), Associate Professor at the Chemistry Department

E. R. Luchkevych, Vasyl Stefanyk Precarpathian National University

PhD (Chemistry), Associate Professor at the Environmental Chemistry and Chemical Education Department

V. I. Shupenyuk, Vasyl Stefanyk Precarpathian National University

PhD (Chemistry), Associate Professor at the Environmental Chemistry and Chemical Education Department

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Published

2024-09-30

How to Cite

Zavhorodnii, M. P., Derevianko, N. P., Shkopynska Т. E., Brazhko, O. A., Kornet, M. M., Gencheva, V. I., … Shupenyuk, V. I. (2024). STUDY OF THE EFFECT OF S-HETEROCYCLIC SUCCINATES ON MICROCLONAL PROPAGATION OF ORNAMENTAL PLANTS. Pharmaceutical Review Farmacevtičnij časopis, (3), 21–34. https://doi.org/10.11603/2312-0967.2024.3.14838

Issue

Section

Pharmacological researches of biologically active substances