THE EFFECT OF MAITAKE MUSHROOMS THICK EXTRACT ON THE SIGNS OF INFLAMMATORY PROCESS UNDER THE CONDITIONS OF TESTOSTERONE-INDUCED BENIGN PROSTATIC HYPERPLASIA IN RATS
DOI:
https://doi.org/10.11603/2312-0967.2023.4.14365Keywords:
maitake extract, prostate hyperplasia, prostate protective effect, anti-inflammatory effectAbstract
The aim of the work. The objective of work was to study the effect of maitake mushrooms dense extract on the development of inflammatory processes in white rats with testosterone-induced benign prostatic hyperplasia.
Materials and Methods. The investigation was carried out on male white rats, randomized into 7 groups of 8 animals each. Benign prostatic hyperplasia was modeled by subcutaneous injection of testosterone propionate at a dose of 3 mg/kg body weight of the animal for 21 days. A thick extract of maitake mushrooms was used to correct the damage caused. The еxtract was given orally each day for a period of three weeks simultaneously with testosterone, with a dosage of 150 mg/kg based on the anіmal's body wеіght. "Prostatofit" was chosen as a reference drug, which was administered intragastrically according to the same scheme as the studied extract in a dilution of 1:10 at a dose of 1 ml/100 g of rat body weight. Rats were euthanized using sodium thiopental on the 15th and 22nd days of experiment. The anti-inflammatory effect of dense extract from maitake mushrooms was evaluated by the following biochemical indicators: the number of leukocytes, the erythrocyte sedimentation rate value, the content of C-reactive protein, interleukin-6, tumour necrosis factor-α, testosterone and dihydrotestosterone in the blood serum of animals.
Results and Discussion. The effect of maitake mushrooms dense extract on the development of prostate adenoma in white rats induced by subcutaneous injection of testosterone propionate was studied. It was determined through experimentation that the subcutaneous administration of testosterone propionate to rats for 3 weeks in order to simulate benign prostatic hyperplasia results in the initiation of an inflammatory process, which is confirmed by a significant increase in the number of leukocytes, erythrocyte sedimentation rate, the content of C-reactive protein, interleukin-6, tumour necrosis factor-α, testosterone and dihydrotestosterone in blood serum of experimental animals. Administration of 150 mg/kg dosage of mаіtаke mushrоom thick еxtrаct in parаllel with testosterone showed a reliable positive effect on the investigated biochemical indicators in all terms of the experiment.
Conclusions. The results acquired affirm the anti-inflammatory properties of the concentrated extract obtained from maitake mushrooms. This supports the idea of exploring its potential as a protector for the prostate and considering its integration into a comprehensive treatment plan for benign prostatic hyperplasia.
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