STUDY OF THE INFLUENCE OF ARTEMISIA TILESII EXTRACT ON THE PROCESS OF FORMATION OF SUPEROXIDE RADICALS IN THE SYSTEM OF AUTO-OXIDATION OF ADRENALINE
DOI:
https://doi.org/10.11603/2312-0967.2023.3.14167Keywords:
“hairy” roots of Artemisia tilesii, adrenaline, reactive oxygen species, flavonoids, active pharmaceutical ingredient, molecular mechanismAbstract
The aim of the work. Study of the effect of water-ethanol (30:70) extract from the “hairy” roots of Artemisia tilesii on the process of formation of superoxide radicals in the redox system of autoxidation of adrenaline.
Materials and Methods. A water-ethanol (30:70) extract from of the «hairy» roots of Artemisia tilesii was obtained in the Laboratory of Adaptive Biotechnology of the Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine.
The total content of flavonoids in the extract was determined by the spectrophotometric method and expressed in routine equivalent.
The study of the effect of the extract from the «hairy» roots of Artemisia tilesii on superoxide radicals, which are generated during autooxidation of adrenaline, was carried out in vitro spectrophotometrically. The quantitative assessment of the process was carried out by calculating the first-order rate constants.
Results and Discussion. It was established that the water-ethanol (30:70) extract from the “hairy” roots of Artemisia tilesii, rich in compounds of flavonoid nature, in the chemical system of autoxidation of adrenaline reliably reveals pro-oxidant properties that depend on its concentration in the system. Already at a concentration of the extract in the system of 50 mkM (according to routine), the rate constant of the chemical reaction of the formation of superoxide radicals increases by 2.3 times.
Conclusions. The obtained results confirm that the extract from the “hairy” roots of Artemisia tilesii in the chemical system of autoxidation of adrenaline exhibits a pro-oxidant effect, stimulating the formation of superoxide radicals. Taking into account the research data, which indicate that the generation of reactive oxygen species and their increased level are directly related to antibacterial activity, it can be assumed that potentially this extract due to its pro-oxidant properties can exhibit an antimicrobial effect and be used as an active pharmaceutical ingredient drugs with antibacterial action.
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