PHARMACOLOGICAL STUDY OF VENOTROPIC ACTIVITY OF DRY EXTRACT OF SAKHALIN WILLOW SHOOTS
DOI:
https://doi.org/10.11603/2312-0967.2023.2.14055Keywords:
DESWS, venotropic activity, anticoagulant activity, rats, escuvit, chronic venous insufficiencyAbstract
The aim of the work. To study the venotropic and anticoagulant activity of the dry extract of Sakhalin willowshoots (DESWS).
Materials and Methods. The anticoagulant activity was determined in an «in vitro» experiment by the Burker method. To further study the anticoagulant activity of DESWS, we proceeded to an «in vivo» experiment, where we used doses of 30 mg/kg, 60 mg/kg, and 90 mg/kg. For 4 days, once a day the animals were administered intragastrically with the studied DESWS in the form of an aqueous solution at the rate of 0.5 ml per 100 g of body weight. On the 4th day, blood was taken from the tail of the rats after the injection of the test samples in 60 min and the blood clotting time was determined. The next stage of our research was to study the effect of DESWS on the course of edema in venous stasis of the tail in rats. The object of the study was a dry extract of Sakhalin willow shoots, conventionally designated DESWS.
Results and Discussion. DESWS was used in three test doses in the form of aqueous solutions. The study was carried out on white non-breeding rats weighing 200–250 g, 5 rats per group: group 1 – intact control, which was administered distilled water; group 2 – rats receiving the test extract at a dose of 20 mg/kg, group 3 – rats receiving DESWS at a dose of 30 mg/kg, group 4 – rats receiving DESWS at a dose of 40 mg/kg. According to the data obtained, DESWS at a dose of 20 mg/kg significantly increased the blood clotting time by 1.15 times compared to the control. The anticoagulant activity of DESWS at a dose of 30 mg/kg and 40 mg/kg was almost at the same level, as evidenced by a significant slowing of blood clotting time compared to the control by 1.47 and 1.5 times, respectively. The comparison drug Escuvit increased the blood clotting time by 1.26 times compared to the control.
Conclusions. DESWS has shown anticoagulant activity in «in vitro» and «in vivo» studies. The conditionally therapeutic dose of DESWS-30 mg/kg was established in the study of anticoagulant activity in animals. In the model of venous stasis in rats, DESWS exhibits anti-edematous, anti-inflammatory, and venotropic effects, which are not inferior in activity to the comparison drug Escuvit.
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