STUDY OF THE ANTI-INFLAMMATORY ACTIVITY OF IBUPROFEN ESTERS IN COMBINATION WITH LOW-FREQUENCY ULTRASOUND ON THE CARRAGINAN INFLAMMATION MODEL
DOI:
https://doi.org/10.11603/2312-0967.2021.4.12635Keywords:
ibuprofen, ibuprofen esters, low-frequency ultrasound, carrageenan, anti-inflammatory activityAbstract
The aim of the work. Study of the anti-inflammatory activity of the ointment with ibuprofen esters in combination with low-frequency ultrasound.
Materials and Methods. The anti-inflammatory effect of the synthesized compounds was evaluated in a model of carrageenan inflammation by subplantar injection of a carrageenan solution into the hind limb of rats. Low-frequency ultrasound with a frequency of 20-25 kHz was used to enhance skin permeability. The study used white male Wistar rats weighing 180–220 g. The dynamics of changes in the inflammatory process was determined by measuring the thickness and volume of the affected limbs. A 5 % ibuprofen ointment "Dolgit cream" was used as a reference drug. Statistical indicators were calculated using the Microsoft Excel software package.
Results and Discussion. According to the results of the study of the volume of the affected limbs, it was found that starting from the 4th day of treatment, ibuprofen ethyl ester in combination with low-frequency ultrasound demonstrates better anti-inflammatory activity than the reference drug. A similar trend is observed when determining the thickness of the affected limb. Іn the group treated with ibuprofen ethyl ether, the thickness of the affected limbs reaches the physiological norm on the 6th day of treatment, while in the group treated with ibuprofen hexadecyl ether – on the 7th day.
Conclusions. The results obtained indicate that the use of synthesized ibuprofen esters in combination with low-frequency ultrasound leads to an increase in their anti-inflammatory activity. The best dynamics of anti-inflammatory activity is inherent in ibuprofen ethyl ester.
References
Lee JW, Gadiraju P, Park J, Allen MG. Microsecond Thermal Ablation of Skin for Transdermal. Drug Delivery. J. Control. Release. 2011 Aug;154(1):58-68.
Ivaschenko SV, Ostapovich AA, Bezzubic SD. Influence of low-frequency pulsed ultrasound on bone tissue. Modern dentistry. 2014 Jan;1(58):90-3.
Sheina AN. Role and place of ultrasound in the rehabilitation treatment of joint disease. Physiotherapy, balneology and rehabilitation. 2013 Jun;5:38-40.
Polat BE, Hart D, Langer R, Blankschtein D. Ultrasound-Mediated Transdermal Drug Delivery: Mechanisms, Scope, and Emerging Trends. J. Control. Release. 2011 Jun;152(3):330-48.
Polat BE, Blankschtein D, Langer R. Low-frequency sonophoresis: application to the transdermal delivery of macromolecules and hydrophilic drugs. Expert Opin Drug Deliv. 2010 Dec;7(12):1415-32.
Haupenthal DPDS, Dias FM, Zaccaron RP, et all. Effects of phonophoresis with ibuprofen associated with gold nanoparticles in animal model of traumatic muscle injury. Eur J Pharm Sci. 2020 Feb 15;143:105120
Ferrante F.M. Nonsteroidal anti-inflammatory drugs. Medicine. 1998. 388 р.
Boureau F, Schneid H, Zeghari N, Wall R, Bourgeois P. The IPSO study: ibuprofen, paracetamol study in osteoarthritis. A randomised comparative clinical study comparing the efficacy and safety of ibuprofen and paracetamol analgesic treatment of osteoarthritis of the knee or hip. Ann Rheum Dis. 2004 Sep;63(9):1028-34.
Kravchenko IA, Kiryeva MA, Alekseyeva YeA. Synthesis and anti-inflammatory activity of esters of ibuprofen. Chimiko-farmatsevtichesky zhurnal 2014;48(5):14-7.
Boyko YuA, Bogomolny RB, Ayat M, Kravchenko IA. Anti-inflammatory properties of new dosage forms of extracts of bitter pepper. Actual problems of biology, ecology, medicine and pharmacology: mater. scientific and practical. conf with int. participation Dnipropetrovsk, 26-27 Sept. 2013 Dnipropetrovsk, 2013. 143.
European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg: Council of Europe. 1986; 123. 51 p.
Kravchenko IA, Andronati SA, Larionov VB. Physico-chemical basis of strengthening the transdermal delivery of drugs, “Astroprint” Odessa. 2002, 238 p.
Kravchenko IA, Kobernik AA, Aleksandrova AI, Prystupa BV, Lepikh I, Shegur PA. Anti-inflammatory action of therapeutic and low-frequency ultrasound on the model of inflammatory process in rats. Biofizika. 2013 May-Jun; 58(3):540-6.
Kravchenko IA, Prystupa BV, Kireva MV, Kobernik AO. Anti-inflammatory activity of ibuprofen esters in a model of aseptic formalin-induced inflammation when administered orally and transdermally. Odessa Medical Journal. 2015;3(149):20-5.
Kravchenko IA, Prystupa BV, Kireva MV, Kobernik AO. Anti-inflammatory activity of new esters of ibuprofen during transdermal administration. Visnyk ONU Ser .: Biology. 2015;20.1(36):171-6.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Pharmaceutical Review / Farmacevtičnij časopis

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The authors who publish their materials in this journal agree with the following terms:
- Authors reserve the right to authorship of their work and assign to the journal the right to first publish this work under the terms of the Creative Commons Attribution License, which allows other persons to freely distribute the published work with a mandatory reference to the authors of original work and the first publication of work in this journal
.
- Authors have the right to make independent extra-exclusive work agreements in the form they are published by this journal (for example, posting work in an electronic repository of an institution or publishing as part of a monograph), provided that the link to the first publication of the work in this journal is maintained.
Journal policy allows and encourages publication of manuscripts on the Internet (for example, in institutions repositories or on personal websites), both before the publication of this manuscript and during its editorial review, as it contributes to productive scientific discussion and positively affects the efficiency and dynamics of the citation of the published work (see The Effect of Open Access).