STUDY OF THE POSSIBILITY OF INDIVIDUAL PLANTS IDENTIFICATION IN A MULTI-COMPONENT HERBAL MEDICINAL PRODUCT
DOI:
https://doi.org/10.11603/2312-0967.2021.3.12477Keywords:
herbal spices, filter bags, burr-marigold herb, calendula flowers, hawthorn leaves and flowers, identification, macroscopy, microscopy, TLCAbstract
The aim of the work. Development of methods for identification of multicomponent plant spices in filter packets by characteristic macro and microscopic features, study of chromatographic profile of raw materials and comparison with the profile of potential spices to identify characteristic zones using the TLC method.
Materials and Methods. The herbal drugs (burr-marigold herb, calendula flowers, hawthorn leaves and flowers) were collected in the Kharkiv region and registered in the State Enterprise "Pharmacopoeial Center". The raw material was grounded to a particle size passing through a sieve of 3000. The work included three stages: at the 1st stage the macroscopic analysis of the mixture of herbal drugs was performed, at the 2nd stage - microscopic analysis, and at the third - TLC analysis of the raw material and the multicomponent spices followed by detection of characteristic zones.
Results and Discussion. Macroscopic and microscopic analysis of the species revealed all the characteristic parts of each of the plants that are part of it. During the TLC test for detecting zones of flavonoids and hydroxycinnamic acids, in the chromatogram of the spices characteristic zones of burr-marigold herb, calendula flowers and hawthorn leaves and flowers were showed, as well as zones of calendulosides which are diagnostic for calendula flowers were identified.
Conclusions. Methods for the identification of a multicomponent herbal medicinal product – spices of the burr-marigold herb, calendula flowers, hawthorn leaves and flowers in filter bags by sections that are mandatory for herbal medicinal products: identification A - macroscopy, identification B - microscopy, identification C - TLC have been developed and the possibility of recognizing all its components have been proved.
References
Gudzenko AV, Tsurkan ОО, Koval’chuk TV. [The domestic market of multicomponent herbal medicines: analysis of the structure and development prospects]. Farm Zh. 2012;1:8-12. Ukrainian. – Available from: http://nbuv.gov.ua/UJRN/pharmazh_2012_1_3.
Rational combinations of medicinal plants in the collection. [Electronic resource]. Ukrainian. – Available from: https://www.bsmu.edu.ua/blog/3642-ratsionalni-kombinatsii-likarskih-roslin-u-zborah/.
Ruban OA, Malynovska SA, Murad Al-Tovaiti, Mazurets SI. [Prospects for the creation of new original drugs based on substances of plant origin]. Phytotherap. Chasop. 2012;2:63-65. Ukrainian.
Kotov S, Gontova T. Precondition to the development of the herbal medicinal product with desensitizing activity. Science and modern pharmaceutical manufacturing: VII Annual scientific and practical conference of Farmak’s School of young scientists with international involvement, Kyiv, 21 November, 2019. Ukr. med. chasop. 2019;2,6(134):12–13.
Kotov SA, Gontova ТM, Kotov AG. [Design aspects of the combined medicinal product with multifunctional action based on herbal drugs]. Farm Zh. 2021;5:57-67. Ukrainian.
Lyapunov NA., Bezuglaya EP. [Modern methodology for pharmaceutical drug development]. Farm. Otrasl. 2013; February 1(36):79-86. Ukrainian.
Shostak TA, Kalinyuk TG, Gudz NI. [Features of pharmaceutical development of herbal preparations]. Phytotherap. Chasop. 2014;4:77-82. Ukrainian.
Vovk OG, Kotov AG. [Morphological and anatomical analysis as an important method of identification and standardization of herbal druds].Theoretical and practical aspects of research of medicinal plants: materials of the I international scientific-practical internet-conference (Kharkiv, March 20-21, 2014). Kharkiv: NUPh Publishing House. 2014:6-7. Ukrainian.
Kotov S. Study of the water extracts composition from the combined herbal tea with antiallergic activity. Modern medicine and pharmaceutics: new approaches and current research: materials of the 75th International scientific-practical conference of medical students and young scientists (Samarkand, May 18, 2021). Journal of Hepato-Gastroenterological Research. 2021;2(1):138-9.
Kotov SA, Kotova EE., Bezruk IV, Gontova TN, Kotov AG. Study of the dynamics extraction of biologically active substances from the bur-marigold herb and antioxidant activity of the obtained extracts. EUREKA: Health Sciences. 2020;6:95-101.
Kotov AG, Garna SV, Kotova EE. et al. [Pharmacopoeial requirements for quality control methods of herbal drugs. Methodical recommendations]. Kharkiv: NUPh, Institute of Advanced Training of Pharmacy Specialists, SE "Pharmacopoeial Center". 2016: 54. Ukrainian.
State Pharmacopoeia of Ukraine 2.5. [Державна фармакопея України 2.5]. Kharkiv: DP «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv»; 2021:324-7. Ukrainian.
State Pharmacopoeia of Ukraine 2.0. In 3 volumes. [Державна фармакопея України 2.0. В 3 т.]. Kharkiv: DP «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv»; 2014: 732. Ukrainian.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Pharmaceutical Review / Farmacevtičnij časopis
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The authors who publish their materials in this journal agree with the following terms:
- Authors reserve the right to authorship of their work and assign to the journal the right to first publish this work under the terms of the Creative Commons Attribution License, which allows other persons to freely distribute the published work with a mandatory reference to the authors of original work and the first publication of work in this journal
. - Authors have the right to make independent extra-exclusive work agreements in the form they are published by this journal (for example, posting work in an electronic repository of an institution or publishing as part of a monograph), provided that the link to the first publication of the work in this journal is maintained.
Journal policy allows and encourages publication of manuscripts on the Internet (for example, in institutions repositories or on personal websites), both before the publication of this manuscript and during its editorial review, as it contributes to productive scientific discussion and positively affects the efficiency and dynamics of the citation of the published work (see The Effect of Open Access).
