CONTROL OF RESIDUAL HYDROGEN PEROXIDE AFTER DISINFECTION OF LABORATORY GLASSWARE AND EQUIPMENT

Authors

  • V. M. Britsun State Institution “O. M. Marzeyev Institute for Public Health of the Na¬tional Academy of Medical Sciences of Ukraine”
  • N. L. Tarasenko State Institution “O. M. Marzeyev Institute for Public Health of the National Academy of Medical Sciences of Ukraine”
  • N. O. Savina State Institution “O. M. Marzeyev Institute for Public Health of the National Academy of Medical Sciences of Ukraine”
  • N. V. Оstanina State Institution “O. M. Marzeyev Institute for Public Health of the National Academy of Medical Sci¬ences of Ukraine”

DOI:

https://doi.org/10.11603/2312-0967.2021.2.12145

Keywords:

hydrogen peroxide, residual amounts, permanganometry, reaction sensitivity, oxidizable substances, conductometry, specific conductance

Abstract

The aim of the work was to determine the residual concentration of hydrogen peroxide in the distilled water which meets the requirements of the State Pharmacopoeia of Ukraine. It seemed necessary to justify the amount of flushing water required for the complete removal of H2O2.

Materials and Methods. Deoinized water was obtained from the "Millipore Direct-Q 3 UV" system, distilled water - from the "GFL2008" aquadistillator. Hydrogen peroxide solutions of 30% (produced by "Carlo Erba Reagents S.A.S", "For analysis ACS-Reag.Ph.Eur.-Reag.USP-Stabilized" qualification) and 35% (produced by "UKRHIM", "medical" qualification) were used. The experiments were carried out using the pharmacopoeial test "oxidizable substances" and conductometric measurements. The conductometric studies were performed on the "Hanna HI 2300" conductometer.

Results and Discussion. It has been established that the use of the pharmacopeia test “oxidizable substances” (permanganometric method) allows the identification of hydrogen peroxide at  concentration of approximately ≥ 10-4%.

The specific conductance (SC) of diluted (3.5х10-5…3.5%)  "pharmacopoeial" and  "medical"  hydrogen peroxide solutions was also measured. It was found that the diluted solutions of H2O2 "pharmacopoeial" and "medical" qualification significantly differ in SC. Probably, this is due to the presence of stabilizers of different nature (organic and inorganic) and in different concentrations (in "pharmacopoeial" H2O2 - in minimal, in "medical" - in high concentration).

Conclusions. It was found that, the concentration of hydrogen peroxide decreases by about 50-100 times with each rinsing of laboratory glassware with distilled water. Therefore, 3-4 times washing with distilled water is needed for complete removal (to reach a concentration <10-4%) of hydrogen peroxide from the laboratory glassware and equipment. Theoretical calculations were confirmed by experimental data.

Thus, hydrogen peroxide has a low SC, which is less than the SC of the stabilizer solutions in it, and the conductometric method is not suitable for controlling residual H2O2 in the aboratory glassware and equipment.

Author Biographies

V. M. Britsun, State Institution “O. M. Marzeyev Institute for Public Health of the Na¬tional Academy of Medical Sciences of Ukraine”

DSс (Chemistry), chief researcher

N. L. Tarasenko, State Institution “O. M. Marzeyev Institute for Public Health of the National Academy of Medical Sciences of Ukraine”

lead engineer

N. O. Savina, State Institution “O. M. Marzeyev Institute for Public Health of the National Academy of Medical Sciences of Ukraine”

scientific researcher

N. V. Оstanina, State Institution “O. M. Marzeyev Institute for Public Health of the National Academy of Medical Sci¬ences of Ukraine”

PhD (Economics), scientific researcher, Head of the State Scientific Research Laboratory for Quality Control of Medicines

References

The State Pharmacopoeia of Ukraine: in 3 vol. Kharkiv: Ukrainian Scientific Pharmacopoeia Cen¬ter of Quality of Medicinal Products. Ed.2 [Derzhavna Farmakopeya Ukrayiny: v 3 t. / DP "Ukrayinsʹkyy naukovyy farmakopeynyy tsentr yakosti likarsʹkykh zasobiv" – 2-e vyd.], Kharkiv: Ukrainian Scientific Pharmacopoeia Cen¬ter of Quality of Medicinal Products, 2014; Ukrainian. Guidelines for drinking-water quality - 4th ed. WHO, 2011, 564 p. Available from: http://apps.who.int/iris/bitstream/handle/10665/44584/9789241548151_eng.pdf;jsessionid=ECE0BAADE0B0AD93BE2B08C72A669805?sequence=1

Yoo JH. Review of Disinfection and Sterilization - Back to the Basics. Infect. Chemother. 2018;50(2):101–9. doi: 10.3947/ic.2018.50.2.101

McDonnel G, Russel AD. Antiseptics and Disinfectants: Activity, action, and resistance. Clinical Microbiology Reviews. 1999;12(1):147-79. doi: 10.1128/CMR.12.1.147

Sunabc Y, Liue H, Tanabc X, Lid Z. Highly efficient redox reaction between potassium permanganate and 3,3′,5,5′-tetramethylbenzidine for application in hydrogen peroxide. RSC Advances. 2019;9:1889-94. doi: 10.1039/C8RA07758D

Hsu CC, Lo YR, Lin YC, Sh YC. A Spectrometric Method for Hydrogen Peroxide Concentration Measurement with a Reusable and Cost-Efficient Sensor. Sensors. 2015;15: 25716-29. doi: 10.3390/s151025716

Klassen NV, Marchington D, McGowan C. H2O2 determination by I3- method and KMnO4 titration. Anal. Chem. 1994;66: 2921-5. doi: 10.1021/ac00090a020

Blazheyevskiy M, Riabko D. Development and validation of the spectrophotometric technique of the peroxide remains detection in washing waters when controlling the quality of sanitary processing of equipment. GISAP Medical Science, Pharmacology. 2016;9:33-8. doi: 10.18007/gisap:msp.v0i9.1270

Patrick WA, Wagner HB. Determination of Hydrogen Peroxide in Small Concentrations. Anal. Chem. 1949;21(10):1279-80. doi: 10.1021/ac60034a038

Meier J, Hoerber EM. , Stapleton JA, Iverson NM. Hydrogen Peroxide Sensors for Biomedical applications. Chemosensors. 2019;7,64. doi: 10.3390/chemosensors7040064

Karyakin A A, Bolshakov IA, Karyakina E.E. Electrochemical Sensor with Record Performance Characteristics. Angew. Chem. 2007;119(40):7822-24. doi: 10.1002/ange.200700341

Schumb WC, Satterfield CN, Wentworth RL. Hydrogen Peroxide. Reinhold publishing corporation: New-York, 1955. doi: 10.1002/jps.3030450224

Sulistyarti H, Atikah A, Fardiyah Q, Febriyanti S. A Simple and Safe Spectrophotometric Method for Iodide Determination. Makara J. Sci. 2015;19/2:43-8. doi: 10.7454/mss.v19i2.4736

Tamres M, Frost A. Adsorption Effects in the Decomposition of Hydrogen Peroxide Vapor. J. Am. Chem. Soc. 1950;72(11):5340-3. doi: 10.1021/ja01167a539

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Published

2021-07-14

How to Cite

Britsun, V. M., Tarasenko, N. L., Savina, N. O., & Оstanina N. V. (2021). CONTROL OF RESIDUAL HYDROGEN PEROXIDE AFTER DISINFECTION OF LABORATORY GLASSWARE AND EQUIPMENT. Pharmaceutical Review Farmacevtičnij časopis, (2), 21–27. https://doi.org/10.11603/2312-0967.2021.2.12145

Issue

No. 2 (2021)

Section

Analysis of drugs

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