CONTROL OF RESIDUAL HYDROGEN PEROXIDE AFTER DISINFECTION OF LABORATORY GLASSWARE AND EQUIPMENT
DOI:
https://doi.org/10.11603/2312-0967.2021.2.12145Keywords:
hydrogen peroxide, residual amounts, permanganometry, reaction sensitivity, oxidizable substances, conductometry, specific conductanceAbstract
The aim of the work was to determine the residual concentration of hydrogen peroxide in the distilled water which meets the requirements of the State Pharmacopoeia of Ukraine. It seemed necessary to justify the amount of flushing water required for the complete removal of H2O2.
Materials and Methods. Deoinized water was obtained from the "Millipore Direct-Q 3 UV" system, distilled water - from the "GFL2008" aquadistillator. Hydrogen peroxide solutions of 30% (produced by "Carlo Erba Reagents S.A.S", "For analysis ACS-Reag.Ph.Eur.-Reag.USP-Stabilized" qualification) and 35% (produced by "UKRHIM", "medical" qualification) were used. The experiments were carried out using the pharmacopoeial test "oxidizable substances" and conductometric measurements. The conductometric studies were performed on the "Hanna HI 2300" conductometer.
Results and Discussion. It has been established that the use of the pharmacopeia test “oxidizable substances” (permanganometric method) allows the identification of hydrogen peroxide at concentration of approximately ≥ 10-4%.
The specific conductance (SC) of diluted (3.5х10-5…3.5%) "pharmacopoeial" and "medical" hydrogen peroxide solutions was also measured. It was found that the diluted solutions of H2O2 "pharmacopoeial" and "medical" qualification significantly differ in SC. Probably, this is due to the presence of stabilizers of different nature (organic and inorganic) and in different concentrations (in "pharmacopoeial" H2O2 - in minimal, in "medical" - in high concentration).
Conclusions. It was found that, the concentration of hydrogen peroxide decreases by about 50-100 times with each rinsing of laboratory glassware with distilled water. Therefore, 3-4 times washing with distilled water is needed for complete removal (to reach a concentration <10-4%) of hydrogen peroxide from the laboratory glassware and equipment. Theoretical calculations were confirmed by experimental data.
Thus, hydrogen peroxide has a low SC, which is less than the SC of the stabilizer solutions in it, and the conductometric method is not suitable for controlling residual H2O2 in the aboratory glassware and equipment.
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