RESEARCH OF ANTIBACTERIAL PROPERTIES OF EXTEMPORANEOUS SUSPENSION WITH BISMUTH NITRATE BASIC
DOI:
https://doi.org/10.11603/2312-0967.2021.1.11939Keywords:
suspension, antibacterial activity, research methodsAbstract
The aim of the work. Research of the antibacterial properties of the extemporaneous suspension with bismuth nitrate basic for the further development of a drug for use in gastroenterology, in particular the treatment of peptic ulcers of the stomach and duodenum.
Materials and Methods. Samples of basic bismuth nitrate suspension, mainly made by traditional technology (method of “making muddy”) and with the adding of a stabilizers were the objects of the examination. For the study of antibacterial properties there were used reference test cultures of gram-positive and gram-negative bacteria belonging to different taxonomic groups, in particular Staphylococcus aureus ATCC 25923, Esсherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Bacillus subtilis ATCC6633, Proteus vulgaris ATCC 4636. The antifungal effect of the samples was investigated on the reference strain of Candida albicans ATCC 885-653.
Results and Discussion. The results of the study show that the sample with the content of excipients has a more pronounced antimicrobial effect on all the studied strains of microorganisms, as evidenced by the values of the diameter of the growth inhibition zones and the exposure time in comparison with the sample prepared according to the traditional technology.
Conclusions. The antibacterial activity of extemporaneous suspension samples against reference test cultures of gram-positive and gram-negative bacteria was established. It was found that the content of excipients in the suspension does not reduce the antibacterial activity of the suspension sample, but also increases it for almost all strains of microorganisms.
References
Marcus EA, Vagin O, Tokhtaeva E, Sachs G, Scott DR. Helicobacter pylori impedes acid-induced tightening of gastric epithelial junctions. Am J Physiol Gastrointest. Liver Physiol. 2013;305: 731-9. DOI: 10.1152/ajpgi.00209.2013
Himion LV, Yashchenko OB, Danilyuk SV, Sityuk TO. [Modern approaches to the diagnosis and manage¬ment of patients with peptic ulcer of the stomach and duodenum at the primary level of care]. Semeynaya meditsina. 2018;1(75): 6-11. Ukrainian.
Grigorev P Ya, Starodub EM, Yakovenko EP. Diseases of the digestive system (diagnosis and treatment): textbook. [Хвороби органів травлення (діагностика і лікування): навч. посіб.] Ternopil: Ukrmedknyha; 2012. Ukrainian.
O'Connor A, Fischbach W, Gisbert JP, O'Morain C. Treatment of Helicobacter pylori infection. Helicobacter. 2016;1: 55-61.
Sugano K, Tack J, Kuipers EJ. Kyoto global consensus report on Helicobacter pylori gastritis. Gut. 2015;64: 1353-67.
Angel Lanas, Francis KL. Chan peptic ulcer disease. The Lancet. 2017;10094: 613-24.
Malfertheiner P, Mégraud F, O’Morain C. European Helicobacter and Microbiota Study Group and Consensus panel. Management of Helicobacter py¬lori infection – the Maastricht V/Florence Consensus Report. Gut. 2017;66(1): 6-30. DOI: 10.1136/ gutjnl-2016-312288
Mendis AHW, Marshall BJ. Helicobacter pylori and bismuth / Biological chemistry of arsenic, antimony and bismuth. Sun H (Ed.). Singapore: John Wiley & Sons Ltd., 2011.
Tsang CN, Ho KS, Sun H, Chan WT. Tracking Bismuth anti-ulcer drug uptake in single Helicobacter pylori cells. J Am Chem Soc. 2011;133(19): 7355-7.
Xia W, Li H, Sun H. Functional disruption of HypB, a GTPase of Helicobacter pylori, by bismuth. Chem Commun (Camb). 2014;50(13): 1611-4.
State Register of Medicines of Ukraine. [Державний реєстр лікарських засобів України] Available from: http://www.drlz.kiev.ua/ Ukrainian.
Compendium Directory [Internet]. Available from: http:// compendium.com.ua.
Yarnykh TG, Tykhonov ОІ, Melnyk GM, Yuryeva GB. Pharmacopoeian aspects of suspensions preparation in pharmacy conditions. Asian Journal of Pharmaceutics. 2017;11(4): 859-64.
Tykhonov OI, Yarnykh TH, Hrytsenko IS, Khokhlenkova NV. Extemporal Formulation (Technology, Analysis, Application): Methodical recommendations. [Екстемпоральна рецептура (технологія, аналіз, застосування) : метод. реком.] Кyiv: Publishing House Agency of Medical Marketing; 2016. Ukrainian.
Voliansky YL, Gritsenko IS, Shirobokov VP et al. The study of the specific activity of antimicrobial drugs: Method. Recom. Kiev. 2004. 38 p. Ukrainian.
State Pharmacopoeia of Ukraine: in 3 volumes. [Державна Фармакопея України: в 3-х т.] Kharkiv: DP “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv”. Ukrainian.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Pharmaceutical Review
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The authors who publish their materials in this journal agree with the following terms:
- Authors reserve the right to authorship of their work and assign to the journal the right to first publish this work under the terms of the Creative Commons Attribution License, which allows other persons to freely distribute the published work with a mandatory reference to the authors of original work and the first publication of work in this journal
. - Authors have the right to make independent extra-exclusive work agreements in the form they are published by this journal (for example, posting work in an electronic repository of an institution or publishing as part of a monograph), provided that the link to the first publication of the work in this journal is maintained.
Journal policy allows and encourages publication of manuscripts on the Internet (for example, in institutions repositories or on personal websites), both before the publication of this manuscript and during its editorial review, as it contributes to productive scientific discussion and positively affects the efficiency and dynamics of the citation of the published work (see The Effect of Open Access).
