SPECTROPHOTOMETRIC DETERMINATION OF ATENOLOL IN TABLETS
DOI:
https://doi.org/10.11603/2312-0967.2021.1.11935Keywords:
atenolol, diazonium salts, spectrophotometry, validationAbstract
The aim of the work. Develop a method for spectrophotometric determination of atenolol with diazonium salts; establish optimal conditions for the quantitative determination of atenolol in drugs; validate the developed methodology.
Materials and Methods. All studies were conducted on the basis of the Experimental Pharmaceutical Research Department of the Scientific Medical Laboratory Center (NMLC) of the Zaporizhzhia State Medical University.
Reagents and solvents used in the present study: diazole red (obtained from NVF "Sinbias"), tablets "Atenolol-Astropharm" 50 mg (LLC "Astropharm", Ukraine, series 050417) and "Atenolol-Astropharm" 100 mg (LLC "Astrofarm" ", Ukraine, series 010218). Methanol (LAB-SCAN, Ireland, batch № 5120/13), sodium carbonate (Sinbias) and distilled water were also used.
Analytical equipment: Spectrophotometer "SPECORD-200" (Analytic Jena AG, Germany), scales laboratory electronic RADWAG XA 210.4Y, bath ultrasonic Sonorex Digitec DT100H, laboratory glassware of class A.
Results and Discussion. The technique of spectrophotometric determination of the quantitative content of atenolol based on its reaction with red diazole in water-methanol medium has been developed. The stoichiometric ratios of the reactive components as 1:1 were obtained by the methods of continuous changes and the saturation method. Validation of the developed on such indicators as linearity, precision, correctness and robustness is carried out. Based on these data, the developed method is correct and can be used in the quality control departments of chemical and pharmaceutical companies.
Conclusions. A method of quantitative spectrophotometric determination of atenolol in the tablet dosage form "Atenolol-Astrapharm" 50 mg and "Atenolol-Astrapharm" 100 mg of industrial production was developed, validation characteristics were investigated: linearity, precision, correctness, range of application and robustness.
References
Compendium. 2021. Directory of drugs Compendium. [Internet] Available at: <https://compendium.com.ua>.
State Pharmacopoeia of Ukraine. 2nd type. [Державна Фармакопея України. 2-е вид.] Kharkiv: State Enterprise "Scientific and Expert Pharmacopoeial Center"; 2015. Ukrainian.
British Pharmacopeia. London: The Stationary Office; 2009.
United States Pharmacopeia. Rockville: USP Convention Inc; 2013.
Dey S, Sarkar S, Malakar J. Spectrophotometric method for simultaneous determination of atenolol and atorvastatin in tablet dosage forms. International Journal of Pharmaceutical and Biomedical Research. 2012;1(3): 40-43.
Yu LL, Liu JC, Li HK. Spectrophotometry determination of atenolol in tablets based on charge transfer complex of atenolol with chloranilic acid. Yaowu Fenxi Zazhi. 2010;3(30): 538-40.
Wislous OA, Bevz NYu, Zhivora NV, Bezugly PA. Quantitative determination of atenolol in Tonorm tablets by spectrophotometry. Journal of Pharmacy. 2014;4 (66): 68-73.
Zhuk YN, Vasyuk SO. Quantitative determination of Atenolol in tablets. IJAPBC. 2015;5(3): 350-4.
Zhuk Y. Spectrophotometric determination of some β-blockers in dosage forms. Proceedings of the 67th International Medical Congress of Students and Young Scientists. Kyiv; 2013. Ukrainian.
Donchenko AA., Vasyuk SO. Spectrophotometric determination of atenolol in dosage forms using 2,3-dichloro-1,4-naphthoquinone. Pharmaceutical Journal. 2017;4: 63-8.
Wislous OO, Bevz NYu, Georgiyants VA. Method of identification and quantification of β-blockers (literature review). Scientific Journal «ScienceRise». 2015;10/4(15): 56-70. Ukrainian.
Akram MEl-Didamony. Application of Bromocresol Green and Bromothymol Blue for the Extractive Spectrophotometric Determination of Anti-hypertensive Drugs. Journal of Applied Pharmaceutical Science. 2015;7(5): 122-9.
Bulatov MI, Kalinkin IP. Practical guide to photometric methods of analysis. 5th ed. [Практическое руководство по фотометрическим методам анализа, 5-е изд.] Leningrad: Chemistry; 1986. Russian.
Grizodub АI. Standardized procedures for validation of methods of quality control of medicines. [Стандартизованные процедуры валидации методик контроля качества лекарственных средств.] Kharkiv: State Enterprise «Ukrainian Scientific Pharmacopoeial Center for Drug Quality»; 2016. Russian.
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