RESEARCH OF ANTIMICROBIAL ACTIVITY OF WATER-ETHANOL EXTRACTS OF AJUGA REPTANS L. HERB

Authors

DOI:

https://doi.org/10.11603/2312-0967.2020.4.11648

Keywords:

Ajuga reptans L., antimicrobial activity, water-ethanol extract, antifungal activity, gram-negative, gram-positive

Abstract

The aim of the work. To determine the microbiological purity of Ajuga reptans L. herb and antimicrobial activity of it’s water-ethanol extracts against clinical strains of opportunistic bacteria and yeast-like fungi.

Materials and Methods. The object of the study was the antimicrobial activity of water-ethanol extracts of Ajuga reptans L. herb, harvested during the period of mass flowering. The study of antimicrobial activity of extracts was performed on clinical isolates of antibiotic-sensitive and antibiotic-resistant microorganisms. Bacterial cultures were identified using biochemical microtests "STAPHYtest 16", "ENTEROtest 24", "NEFERMENTtest 24" (Lachema, Czech Republic), as well as taking into account a set of morphological and cultural properties according to the recommendations of the 9th edition of “Bergey’s Manual of Systematic Bacteriology”. Yeast-like fungal cultures were identified on the basis of 40 biochemical tests using the VITEK 2 system with VITEK 2 YST ID card (Biomerieux, France). The antimicrobial activity of the extracts was studied by the micromethod of diffusion into agar.

Results and Discussion. Screening of antimicrobial properties of water-ethanol extracts (40 %, 50 %, 70 %, 90 %) of Ajuga reptans L. herb was carried out and microbiological purity of herbal extracts was investigated on 25 test strains of microorganisms.

Conclusions. Results of the research indicate that further detailed studies of antimicrobial activity of Ajuga reptans L. herb are promising to increase the range of antimicrobial herbal medicines.

Author Biographies

S. V. Maliuvanchuk, Ivano-Frankivsk National Medical University

Assistant Professor of Pharmacy Department

R. V. Kutsyk, Ivano-Frankivsk National Medical University

DSc (Medicine), Professor of the Microbiology, Virology and Immunology Department

A. R. Grytsyk, Ivano-Frankivsk National Medical University

DSc (Pharmacy), Professor of the Pharmacy Department

References

Terninko II, Vitokhina NV, Shtepa SYu. [Creation of a complex tincture of medicinal plant raw materials and the study of its antimicrobial activity]. Ukrainskyi zhurnal klinichnoi i laboratornoi medytsyny. 2008;3(2). Ukrainian.

Hrodzinskyi AM. Medicinal plants: encyclopedic reference book [Лікарські рослини : енциклопедичний довідник]. Grodzinsky AM ed. Kyiv: Head. ed. URE; 1990. Ukrainian.

Flora of the USSR [Флора УРСР]. Zerov DK. ed. Kyiv: Publishing House AS USSR; 1960: 184-94. Ukrainian.

Atanasov AG, Waltenberger B, Pferschy-Wenzig EM, Linder T, Wawrosch C, Uhrin P, et al. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnol Adv. 2015;33(8):1582-614.

Rauca V-F, Vlase L, Casian T, Sesarman A, Gheldiu A-M, Mocan A, Banciu M [et al.]. Biologically active Ajuga species extracts modulate supportive processes for cancer cell development. Front Pharmacol. 2019;10:334. DOI:10.3389/fphar.2019.00334.

Luan F, Han K, Li M, Zhang T, Liu D, Yu L, Lv H. Ethnomedicinal uses, phytochemistry, pharmacology, and toxicology of species from the Ajuga genus L.: a systematic review. Am J Chin Med. 2019;47(5):959-1003. DOI: 10.1142/S0192415X19500502.

Cocquyt K, Cos P, Herdewijn P, Maes L, Van den Steen PE, Laekeman G. Ajuga remota Benth : from ethnopharmacology to phytomedical perspective in the treatment of malaria. Phytomedicine. 2011;18(14):1229-37. DOI: 10.1016/j.phymed.2011.08.063.

Kuria KAM, Chepkwony H, Govaerts C, Roets E, Busson R, De Witte P, Istvan Zupko [et al.]. The antiplasmodial activity of isolates from Ajuga remota. J Nat Prod. 2002;65(5):789-93. DOI: 10.1021/np0104626.

Alekseyeva LI, Lafon R, Volodin VV, Luksha VG. [Ecdysteroids Ajuga reptans L.]. Fiziologiya rasteniy. 1998;45(3):372-7. Russian.

Alekseyeva LI, Teteryuk LV, Volodin VV, Kolegova NA. [Dynamics of ecdysteroid content in Ajuga reptans L. on the northern border of its range (Komi Republic)]. Rastitelnyye resursy. 1998;34(4):56-62. Russian.

Maliuvanchuk SV, Hrytsyk AR, Melnyk MV. [Investigation of morphological and anatomical features of Ajuga reptans L. grass]. Med Clin Chem. 2018;20(4):120-4. Ukrainian.

Nikirsa TD. Flora of Khotyn upland (Prut-Dniester interfluve): analysis, comparative characteristics and protection. Extended abstract of Candidate’s thesis. Kyiv: NAS Ukraine; 2007. Ukrainian.

Novosad VV, Krytska LI, Lyubinska LH. Phytobiota of the Podilsky Tovtry National Nature Park. Vascular plants. [Фітобіота національного природного парку «Подільські Товтри». Судинні рослини] Kyiv: Fiton; 2009. Ukrainian.

Flora and vegetation of the tract "Pagur" (Transnistrian Podillya) [Electronic resource]. Available from: https://smekni.com/a/9197/flora-roslinnst-urochishcha-pagur/ Ukrainian.

Shanayda MI. [Botanical and pharmacognostic aspects of the study of medicinal plants of the Lamiaceae Juss family]. Fitoterapiia. Chasopys. 2005;2: 50-7. Ukrainian.

Annamukhammedova OO, Annamukhammedov AO. Medicinal plants in tables and diagrams: Textbook. [Лікарські рослини в таблицях та схемах: навч. пос.]. Zhytomyr: ZhSU Publishing House. I. Franko; 2016. Ukrainian.

The State Pharmacopoeia of Ukraine. Kharkiv: Ukrainian Scientific Pharmacopoeial Center for Medicinal Products Quality. [Державна Фармакопея України / ДП «Український науковий фармакопейний центр якості лікарських засобів»]. Kharkiv: Ukrainian Scientific Pharmacopoeial Center for Medicinal Products Quality; 2018. Ukrainian.

Hoult J, Krieg N, Snit P, Staley J, Williams S. eds. Keys to bacteria Berzhdi: in 2 vols. Ed. 9. Transl. from En. [Определитель бактерий Бержди : в 2 т. Изд. 9. Пер. с анг.]. Moscow: Mir; 1997. Russian.

Kutsyk RV. [Study of antimicrobial activity of medicinal plants of Prykarpattia against antibiotic-resistant clinical strains of staphylococci]. Galician Med Bull. 2014;11(4): 44-8. Ukrainian.

Yurchyshyn OI, Kurovets LM, Rusko HV. [Study of antimicrobial and antibiotic-potentiating properties of alcoholic plant extracts against skin isolates of staphylococci - pathogens of pyoderma with different mechanisms of MLS-resistance]. Biomedical and Biosocial Anthropology. 2016;26:52-7. Ukrainian.

Toiu A, Mocan A, Vlase L, Parvu AE, Vodnar DC, Gheldiu AM, Moldovan C. [et al.]. Comparative phytochemical profile, antioxidant, antimicrobial and in vivo anti-inflammatory activity of different extracts of traditionally used Romanian Ajuga genevensis L. and A. reptans L. (Lamiaceae). Molecules. 2019;24(8):1597. DOI: 10.3390/molecules24081597.

Yildirim AB, Karakas FP, Turker AU. In vitro antibacterial and antitumor activities of some medicinal plant extracts, growing in Turkey. Asian Pac J Trop Med. 2013;6(8): 616-24. DOI: 10.1016/S1995-7645(13)60106-6.

Toiu A, Mocan A,Vlase L, Pârvu AE,Vodnar DC, Gheldiu A-M, Moldovan C. [et al.]. Phytochemical composition, antioxidant, antimicrobial and in Vivo anti-inflammatory activity of traditionally used romanian Ajuga laxmannii (Murray) Benth. ("Nobleman’s Beard" - Barba Împăratului). Front Pharmacol. 2018;9:7. DOI: 10.3389/fphar.2018.00007.

Saakyan NZh, Petrosyan MT, Agadzhanyan DzhA. [Antibacterial activity of an isolated culture of the ajuga genevensis]. Biolog. zhurn. Armenii. 2008;1-2:60-5.

Medjeldi S, Bouslama L, Benabdallah A, Essid R, Haou S, Elkahoui S. Biological activities, and phytocompounds of northwest Algeria Ajuga iva L. extracts: Partial identification of the antibacterial fraction. Microb Pathog. 2018;121:173-8. DOI: 10.1016/j.micpath.2018.05.022.

Ben Mansoura M, Balti R, Rabaoui L, Bougatef A, Guerfel M. Chemical composition, angiotensin I-converting enzyme (ACE) inhibitory, antioxidant and antimicrobial activities of the essential oil from south Tunisian Ajuga pseudoiva Rob. L. Proc Biochem. 2013:48:723-9. DOI: 10.1016/j.procbio.2013.02.022.

Wright GD. Opportunities for natural products in 21(st) century antibiotic discovery. Nat Prod Rep. 2017;34(7):694-701. DOI: 10.1039/c7np00019g.

Gibbons S. Anti-staphylococcal plant natural products. Nat Prod Rep. 2004;21(2):263-77. DOI: 10.1039/b212695h.

Kariba RM. Antifungal activity of Ajuga remota. Fitoterapia. 2001;72(2):177-8. DOI: 10.1016/s0367-326x(00)00280-x.

Carbonell P, Coll J. Ajugatansins, neo-clerodane diterpenes from Ajuga reptans. Phytochem Anal. 2001;12(1):73-8.

Ono M, Furusawa C, Ozono T, Oda K, Yasuda S, Okawa M, et al. Four new iridoid glucosides from Ajuga reptans. Chem Pharm Bull (Tokyo). 2011;59(8):1065-8. DOI:10.1248/cpb.59.1065.

Published

2020-11-30

How to Cite

Maliuvanchuk, S. V., Kutsyk, R. V., & Grytsyk, A. R. (2020). RESEARCH OF ANTIMICROBIAL ACTIVITY OF WATER-ETHANOL EXTRACTS OF AJUGA REPTANS L. HERB. Pharmaceutical Review Farmacevtičnij časopis, (4), 63–72. https://doi.org/10.11603/2312-0967.2020.4.11648

Issue

Section

Pharmacological researches of biologically active substances