RESEARCH OF ANTIMICROBIAL ACTIVITY OF WATER-ETHANOL EXTRACTS OF AJUGA REPTANS L. HERB
DOI:
https://doi.org/10.11603/2312-0967.2020.4.11648Keywords:
Ajuga reptans L., antimicrobial activity, water-ethanol extract, antifungal activity, gram-negative, gram-positiveAbstract
The aim of the work. To determine the microbiological purity of Ajuga reptans L. herb and antimicrobial activity of it’s water-ethanol extracts against clinical strains of opportunistic bacteria and yeast-like fungi.
Materials and Methods. The object of the study was the antimicrobial activity of water-ethanol extracts of Ajuga reptans L. herb, harvested during the period of mass flowering. The study of antimicrobial activity of extracts was performed on clinical isolates of antibiotic-sensitive and antibiotic-resistant microorganisms. Bacterial cultures were identified using biochemical microtests "STAPHYtest 16", "ENTEROtest 24", "NEFERMENTtest 24" (Lachema, Czech Republic), as well as taking into account a set of morphological and cultural properties according to the recommendations of the 9th edition of “Bergey’s Manual of Systematic Bacteriology”. Yeast-like fungal cultures were identified on the basis of 40 biochemical tests using the VITEK 2 system with VITEK 2 YST ID card (Biomerieux, France). The antimicrobial activity of the extracts was studied by the micromethod of diffusion into agar.
Results and Discussion. Screening of antimicrobial properties of water-ethanol extracts (40 %, 50 %, 70 %, 90 %) of Ajuga reptans L. herb was carried out and microbiological purity of herbal extracts was investigated on 25 test strains of microorganisms.
Conclusions. Results of the research indicate that further detailed studies of antimicrobial activity of Ajuga reptans L. herb are promising to increase the range of antimicrobial herbal medicines.
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