INVESTIGATION OF PHENOLIC COMPOUNDS OF CAMELINA SATIVA (L.) CRANTZ AND CAMELINA MICROCARPA ANDRZ.
DOI:
https://doi.org/10.11603/2312-0967.2020.4.11539Keywords:
Camelina sativa (L.) Crantz, Camelina microcarpa Andrz., flavonoids, hydroxycinnamic acids, polyphenolic compounds, thin layer chromatography, high performance liquid chromatographyAbstract
The aim of the work. To investigate the composition of phenolic compounds in the herb and seeds of Camelila Crantz speciess.
Materials and Methods. The herb and seeds of the Slavutych C. sativa (L.) Crantz and C. microcarpa Andrz. were used for the study. Water-alcohol extracts were prepared for the studies, and 70 % ethyl alcohol was used as the extragent. For preliminary evaluation of the qualitative composition of extracts, a generally accepted qualitative reactions and thin layer chromatography (TLC) in a system of solvents n-butanol – glacial acetic acid – water (4: 1: 2) with reliable samples of phenolic compounds were used. Chromatograms were examined in daylight and UV light before and after treatment with ammonia vapor. Also, the determination of the qualitative composition and quantitative content of individual phenolic compounds was performed by the method of high performance liquid chromatography (HPLC). Quantitative determination of flavonoids, hydroxycinnamic acids and phenolic compounds was also performed with spectrophotometric method.
Results and Discussion. As a result of TLC, the presence of the substance of flavonoid nature (rutin) in herb and seeds of all samples was established. Hydroxycinnamic acid (chlorogenic acid) has also been identified in the grass of both species. In addition, the HPLC method confirmed the presence of these compounds of phenolic nature in the raw material of the genus Camelina (L.) Crantz. The highest content of rutin was found in the seeds of C. microcarpa Andrz. (0.361±0.001) %, and the highest content of chlorogenic acid – in the herb of C. microcarpa Andrz. (0.279±0.004) %. As a result of spectrophotometric study of the phenolic compounds content in these species raw materials, it was found that the highest content of flavonoids, derivants of hydroxycinnamic acids, and the content of phenolic compounds are characteristic of C. sativa (L.) Crantz herb, and it is (1.17±0.08) %, (1.47±0.03) % and (2.11±0.003) %, respectively.
Conclusions. The TLC method identified and HLCP method quantified the content of rutin and chlorogenic acid in grass and seeds of Camelina sativa (L.) Crantz and Camelina microcarpa Andrz. The total amount of flavonoids, hydroxycinnamic acids and polyphenols in the studied raw materials of both species was determined by spectrophotometric method.
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