STUDYING OF ACUTE TOXICITY AND ANTI-INFLAMMATORY ACTIVITY OF PULSATILA ALBA ETHANOLIC EXTRACTS

Authors

DOI:

https://doi.org/10.11603/2312-0967.2019.2.10189

Keywords:

Pulsatila alba, acute toxicity, anti-inflammatory activity, carrageenan oedema

Abstract

The aim of the work. Research of the acute toxicity and anti-inflammatory (anti-exudative) activity studying of Pulsatila alba ethanolic extracts.

Materials and Methods. Acute toxicity studies were performed on 60 white rats of both sexes weighing 190–220 g, which were administered intragastric single-dose of tested extracts at doses of 5000, 10,000 and 15,000 mg/kg. The carragenine model of inflammatory oedema of white rats paw was used for anti-exudative activity studying. The tested extracts were administered intragastrically at a dose of 2 ml/kg. Diclofenac sodium and ketanov were used as reference drugs.

Results and Discussion. The results of Pulsatila alba ethanolic extracts acute toxicity studying demonstrated absence of any toxic manifestations in intragastric administration at doses of 5000, 10,000 and 15,000 mg/kg in white rats, which allows their to VI class of toxicity (relatively harmless substances). The Pulsatila alba ethanolic extracts posses a tendency for anti-inflammatory activity in the carrageenan model of inflammatory edema in white rats and the pharmacological effect depends on the concentration of ethanol and the ratio of raw material: extractant. The best anti-exudative activity was detected for 70 % ethanolic extracts (1:20), an inhibition index of inflammatory response was 27 %, which is slightly inferior to reference drugs (by 10 and 17 % relative ketanov and sodium diclofenac, respectively) and is a good level for anti-inflammatory phitopreparations.

Conclusions. The acute toxicity and anti-inflammatory effect of Pulsatila alba ethanolic extracts were investigated and it was established that 70 % alcoholic extract (1:20) is a relatively harmless substance (grade VI toxicity) and has potency anti-inflammatory activity (inhibition of inflammation - 27 %) on the carrageenan model of inflammatory edema of the white rat paws at intragastric administration.

Author Biographies

O. S. Khropot, Lviv Polytechnic National University

PhD student, the Technology of Biologically Active Substances, Pharmacy and Biotechnology Department

Yu. T. Konechnyi, Danylo Halytsky Lviv National Medical University

PhD student, the Microbiology Department

Y. I. Kolb, Lviv Polytechnic National University

PhD student, the Technology of Biologically Active Substances, Pharmacy and Biotechnology Department

R. T. Konechna, Lviv Polytechnic National University

PhD (Pharmacy), Associate Professor, the Technology of Biologically Active Substances, Pharmacy and Biotechnology Department

I. І. Hubytska, Lviv Polytechnic National University

PhD (Сhemistry), Associate Professor, the Technology of Biologically Active Substances, Pharmacy and Biotechnology Department

S. M. Holota, Danylo Halytsky Lviv National Medical University

PhD (Pharmacy), Assistant at the Pharmaceutical, Organic and Bioorganic Chemistry Department

O. В. Poshyvak, Danylo Halytsky Lviv National Medical University

PhD (Medicine), Associate Professor at the Pharmacology Department

I. O. Nektehaev, Danylo Halytsky Lviv National Medical University

Senior laboratory assistant, the Pharmacology Department

O. R. Pinyazhko, Danylo Halytsky Lviv National Medical University

DS (Medicine), Professor, Chief of Pharmacology Department

V. P. Novikov, Lviv Polytechnic National University

DS (Сhemistry), Professor, Head of the Technology of Biologically Active Substances, Pharmacy and Biotechnology Department 

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Published

2019-06-12

How to Cite

Khropot, O. S., Konechnyi, Y. T., Kolb, Y. I., Konechna, R. T., Hubytska I. І., Holota, S. M., … Novikov, V. P. (2019). STUDYING OF ACUTE TOXICITY AND ANTI-INFLAMMATORY ACTIVITY OF PULSATILA ALBA ETHANOLIC EXTRACTS. Pharmaceutical Review Farmacevtičnij časopis, (2), 60–66. https://doi.org/10.11603/2312-0967.2019.2.10189

Issue

No. 2 (2019)

Section

Pharmacological researches of biologically active substances

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