АНТИГЕРПЕТИЧНА ЕФЕКТИВНІСТЬ ПРОБІОТИЧНИХ ШТАМІВ ЛАКТО- ТА БІФІДОБАКТЕРІЙ ЗА ЕКСПЕРИМЕНТАЛЬНОГО ГЕНІТАЛЬНОГО ГЕРПЕСУ У МУРЧАКІВ

  • D. B. Starosyla Public Institution L.V. Gromashevskii Institute of Epidemiology and Infectious Diseases of National Academy of Medical Sciences of Ukraine,
  • L. M. Lazarenko D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine,
  • S.M. Hryhorieva Public Institution L.V. Gromashevskii Institute of Epidemiology and Infectious Diseases of National Academy of Medical Sciences of Ukraine
  • L. P. Babenko Інститут мікробіології і вірусології ім. Д.К. Заболотного НАН України
  • S. L. Rybalko ДУ «Інститут епідеміології та інфекційних хвороб ім. Л.В. Громашевського НАМН України»
  • M. Ya. Spivak Інститут мікробіології і вірусології ім. Д.К. Заболотного НАН України, ТОВ «Діапроф» (Київ)
Ключові слова: віруси простого герпесу 2-го типу, лактобактерії, біфідобактерії, генітальний герпес, мурчаки

Анотація

Aim. The aim was to determine the antiherpetic effectiveness of the Lactobacillus casei IMV B-7280 probiotic strain and L. caseі ІМV В-7280 – Bifidobacterium animalis VKL – B. animalis VKВ (В-7280 – VKL – VKВ) composition on the experimental model of genital herpes in guinea pigs induced by herpes simplex virus type 2 (HSV-2).

Materials and methods. Genital herpetic infection was modeled using HSV-2 (strain BH) on female non-breeding guinea pigs. The criteria for assessing the severity of the course of the infectious process were: the area and the degree of specific lesions, as well as the presence of edema, hyperemia and ulcers. HSV-2 titer was determined in blood serum by using common virological methods of research. The spectrum of microbiota in the vagina of animals was determined using generally accepted microbiological methods. The effectiveness of the probiotic bacteria was evaluated during the maximum development of the pathological process: the decrease in the severity of clinical manifestations of the disease and TCD ID50 infectious titer of HSV-2, as well as the reduction of terms of the disease and therapeutic index (TI).

Results and discussion. It was established that in cases of experimental genital herpes in guinea pigs, treatment with L. casei IMV B-7280 probiotic strain or B-7280 - VKL - VKB composition reduced: the severity of clinical symptoms of the disease, the duration of its course, and the infectious titer of HSV-2, though TI did not exceed 50 %. Under the influence of L. casei IMV B-7280 and the composition, there was a decrease in the TCD ID50 infectious titer of HSV-2, which indicates the anti-herpetic efficacy of these probiotic strains, whose mechanisms may involve the change of vaginal microbiome. The microbiological parameters of the vagina at the maximum severity of clinical symptoms of genital herpes were characterized by a decrease in the number of staphylococci in the vagina of infected guinea pigs treated with L. casei IMV B-7280 with a high level of these bacteria after treating of guinea pigs with B-7280 – VKL – VKB composition, as well as lactobacilli (LAB) appearance in individual animals. It should be noted that LAB detected by us at the end of the experiment, differed from the lactobacilli used in the experiment by morphological characteristics. So, we can assume that there has been a restoration of the normal vagina microbiota specific for guinea pigs.

Conclusions. L. casei IMV B-7280 strain and B-7280 – VKL – VKB composition are promising for the development of target probiotics for the prevention and treatment of infectious-inflammatory diseases of the genitourinary system.

Біографії авторів

D. B. Starosyla, Public Institution L.V. Gromashevskii Institute of Epidemiology and Infectious Diseases of National Academy of Medical Sciences of Ukraine,

senior researcher; PhD (Biology), Public Institution L.V. Hromashevskyi Institute of Epidemiology and Infectious Diseases of the National Academy of Medical Sciences of Ukraine, Laboratory of Experimental Chemotherapy of Viral Infections, senior researcher

L. M. Lazarenko, D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine,

senior researcher; DSc (Biology), D.K. Zabolotnyi Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine; leading researcher

S.M. Hryhorieva, Public Institution L.V. Gromashevskii Institute of Epidemiology and Infectious Diseases of National Academy of Medical Sciences of Ukraine

researcher, PhD (Medicine), L.V. Hromashevskyi Institute of Epidemiology and Infectious Diseases of the National Academy of Medical Sciences of Ukraine

L. P. Babenko, Інститут мікробіології і вірусології ім. Д.К. Заболотного НАН України

researcher, PhD (Biology), D.K. Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine

S. L. Rybalko, ДУ «Інститут епідеміології та інфекційних хвороб ім. Л.В. Громашевського НАМН України»

DSc (medicine), Professor, head of the Laboratory of Experimental Chemotherapy of Viral Infections; Public Institution L.V. Hromashevskyi Institute of Epidemiology and Infectious Diseases of National Academy of Medical Sciences of Ukraine

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Опубліковано
2019-07-30
Як цитувати
Starosyla, D. B., Lazarenko, L. M., Hryhorieva, S., Babenko, L. P., Rybalko, S. L., & Spivak, M. Y. (2019). АНТИГЕРПЕТИЧНА ЕФЕКТИВНІСТЬ ПРОБІОТИЧНИХ ШТАМІВ ЛАКТО- ТА БІФІДОБАКТЕРІЙ ЗА ЕКСПЕРИМЕНТАЛЬНОГО ГЕНІТАЛЬНОГО ГЕРПЕСУ У МУРЧАКІВ . Інфекційні хвороби, (2), 34-44. https://doi.org/10.11603/1681-2727.2019.2.10324
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