CLINICAL FEATURES OF THE IMMUNOHISTOCHEMICAL DIAGNOSIS OF BREAST SARCOMAS AND PHYLLODES TUMOURS
DOI:
https://doi.org/10.11603/24116-4944.2017.2.8036Keywords:
breast sarcomas, phyllodes tumors, immunohistochemical diagnosis.Abstract
The aim of the study – to learn the immunohistochemical (IHC) features of breast sarcomas and phyllodes tumours.
Materials and Methods. For IHC study we selected 60 paraffin blocks of tumors, which were histologically verified diagnosis of breast sarcoma (42) and phyllodes tumour (18). IHC study was performed using the spectrum of the corresponding antibodies, which included markers of pan-cytokeratin (clone AE1/AE3); ERG (clone EP111); SOX-10 (clone EP268); TLE1 (clone 1F5); caldesmon (clone h-CD); myogenin (Myf-4) (clone F5D); MyoD1 (clone EP212); desmin (clone D33); MDM2 (1B10); CDK4 (clone EP180); CD68 (clone PG-M1); CD34 (clone QBEnd 10); CD31 (clone JC70A); Beta-catenin (clone beta-Catenin-1); SMA (clone 1A4); actin (clone HHF35); EMA, (MUC1) (clon E29). Also, we determined the level of expression of the marker of proliferation Ki-67 (clone MIB-1). The study was performed in the laboratory CSD Health Care, Kyiv.
Results and Discussion. After performed immunohistochemical studies it was found that with 12 sarcomas without specified histotype in 9 cases there were pleomorphic sarcomas, 2 myofibroblastic sarcomas, and one osteosarcoma. Amond 27 blocks of angiomas, 17 (63.0 %) were immunohistochemically confirmed, and the remaining 10 (37.0 %) were carcinomas. Fibrosarcoma (three histological blocks) were confirmed immunohistochemically. Among 18 histological blocks of borderline phylloid tumors, in two cases (11.1 %) a malignant variant of PT was established.
Conclusions. Immunohistochemical studies play an important role in the differential diagnosis and determination of the histotype of breast sarcomas and the establishment of an accurate diagnosis of phylloid tumors. A diagnostic algorithm is proposed that includes the necessary IHC markers as the base panel for IHC studies, both in the case of BS and in malignant PT.
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