ALLELIC GSTS GENE POLYMORPHISM IN MOTHERS WITH PLACENTAL DYSFUNCTION AND THEIR NEWBORNS: OBSTETRIC AND PERINATAL OUTCOMES
DOI:
https://doi.org/10.11603/2415-8798.2017.1.7464Keywords:
placental dysfunction, genes polymorphism GSTS, intrauterine growth retardation.Abstract
Summary. The role of the placenta caused by the implementation of a number of functional mechanisms of growth and fetal development. Glutathione-S-transferase is actively involved in the removal of products peroxidation lipid peroxides and DNA, restoring organic hydroperoxide to alcohol and contributing isomerization some steroids and prostaglandins. If there are certain polymorphic variants GSTT1, GSTM1, GSTP1 gene family of glutathione-S-transferase is hlutation dependent AOD depletion and inhibition of detoxification function of the placenta.
The aim of the study – to determine the allelic polymorphism gene glutathione-S-transferase GSTT1 and GSTM1 in women with placental dysfunction in healthy newborns and infants with intrauterine growth retardation conducted molecular genetic study of umbilical cord blood.
Materials and Methods. The study involved 105 women; the main group included 33 women with placental dysfunction (PD) without intrauterine growth retardation (IUGR) in children born by them (I group) and 17 women with IUGR, and PD (II group). The control group consisted of 55 women (III group) who gave birth to healthy term infants.
Results and Discussion. It is established that the risk of PD and PD with IUGR by the dominant model of inheritance (313AG + 313GG versus 313AA) significantly increased. The promising prognostication models of intergenic interaction for the assessment of the development of placental dysfunction imply the analysis of GSTM1 and GSTP1 gene polymorphisms and for the assessment of the development of placental dysfunction with intrauterine growth retardation – of GSTT1, GSTM1 and GSTP1. An analysis of serum samples of umbilical cord blood and obtained data revealed that the frequency of GSTM1 polymorphic variant "+" in the field of newborns was 42.69 % and GSTM1 "–" – 57.30 %. The frequency of polymorphic variants GSTT1 «+» in all newborns region was 79.77 % and GSTT1 “–“ – 20.22 %.
Conclusions. The study of the distribution of different allelic variants of genes GSTM1 and GSTT1 proved their differences in different groups of newborns. We found significant difference between the frequencies of GSTM1 gene deletion variant in healthy infants and newborns with IUGR. A pooled analysis of the combined impact of several factors found that pregnancy in women with PD, preeclampsia and GSTM1deletion genotype is associated with a significant increased risk of IUGR fetuses.
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