GENETIC MARKERS OF ANTIOXIDANT PROTECTION IN CHILDREN BORN AFTER ASSISTED REPRODUCTIVE TECHNOLOGIES
DOI:
https://doi.org/10.11603/24116-4944.2017.1.7585Keywords:
children, assisted reproductive technologies, genetic studies, mutation, catalase, superoxide dismutase, hypoxic-induced factor.Abstract
The aim of the study – to examine the effectiveness and evaluate the possibilities of using genetic methods for studying enzymes responsible for antioxidant protection in children born after ART. And also to determine the possible impact of the findings on the health status of this group of children.Materials and Methods. The research analyses the examination of 45 children of the main group, born after assisted reproductive technologies (ART) and 20 children of the control group – born after spontaneous pregnancy (SP). To assess the state of the antioxidant system (AOS), genes that determine the activity of the most important AOS enzymes: SOD-2 (superoxide dismutase) located directly in the mitochondria, and CAT (Catalase). We also thought it appropriate to evaluate the state of HIF (hypoxic-induced factor).
Results and Discussion. In children born after ART, heterozygous mutations of DNA fragments responsible for the functional state of SOD-2 (x2 = 4.68, p = 0.030) and heterozygous and homogeneous mutations in the ratio of CAT (x2 = 3.85, p = 0.049) were observed more often. Mutations responsible for the functioning of CAT, SOD-2 are prognostically unfavorable factors, predictors of more frequent development of respiratory diseases, delay in physical development.Conclusions. The use of genetic studies aimed at studying the function of catalase and superoxide dismutase in children born after ART was effective and useful for further study of the state of health.
References
Desmyttere, S., De Rycke, M., Staessen, C., Liebaers, I., De Schrijver, F., & Verpoest, W. (2012). Neonatal follow-up of 995 consecutively born children after embryo biopsyfor PGD. Human Reproduction, 1, (27), 288–293.
Kimura, N., Tsunoda, S., & Iuchi, Y. (2010). Intrinsic oxidative stress causes either 2-cell arrest or cell death depending on developmental stage of the embryos from SOD1-deficient mice. Molecular Human Reproduction, (16), 441–451.
Komatsu, K., Iwase, A., Mawatari, M., Wang, J., Yamashita, M., & Kikkawa, F. (2014). Mitochondrial membrane potential in 2-cell stage embryos correlates with the success of preimplantation development. Reproduction, 1, (147), 627–638.
Ricci, G., Bogatti, P., Fischer-Tamaro, L.E., Giolo, E, Luppi, S., Montico, M. & (2011). Factor V Leiden and prothrombin gene G20210A mutation and in vitro fertilization: prospective cohort study. Human Reproduction, 11, (26), 3068–3077.
Richard,T., Scott, Jr., Nathan, R. Treff, Stevens, John, Giolo, E., Luppi, S., & Montico, M. (2012). Delivery of a chromosomally normal child from an oocyte with reciprocal aneuploid polar bodies. Assist Reprod Genet, 29, 533–537.
Rita, C., Figueira, S., S. Setti, Amanda, S. Cortezzi, Sylvia, D. Martinhago, Ciro, P. Daniela, A., & Braga, F. (2012). Assumpto Iaconelli Jr.Preimplantation diagnosis for β-thalassemia combined with HLA matching: first “savior sibling” is born after embryo selection in Brazil. Assist Reprod Genet, 29, 1305–1309.
Ruiz-Sanz, J.I., Aurrekoetxea, I., Matorras, R., & Ruiz-Larrea, M.B. (2011). Ala16Val SOD2 polymorphism is associated with higher pregnancy rates in in vitro fertilization cycles. Fertil Steril, 5, (95), 1601–1605.
Zheng, H-Y., Shi, X-Y, Wang, L-L., Wu, Ya-Qin, Chen, Shi-Ling, Zhang, & Lin (2011). Study of DNA methylation patterns of imprinted genes in children born after assisted reproductive technologies reveals no imprinting errors: A pilot study. Experimental and therapeutic medicine, (2), 751–755.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish in this journal agree to the following terms:
1. The authors reserve the right to authorship of the work and pass the journal right of first publication of this work is licensed under a Creative Commons Attribution License, which allows others to freely distribute the work published with reference to the authors of the original work and the first publication of this magazine.
2. Authors are entitled to enter into a separate agreement on additional non-exclusive distribution of work in the form in which it was published in the magazine (eg work place in the electronic repository institution or publish monographs in part), provided that the reference to the first publication of this magazine.
3. Policy magazine allows and encourages authors placement on the Internet (eg, in storage facilities or on personal websites) manuscript of how to submit the manuscript to the editor and during his editorial processing, since it contributes to productive scientific discussion and positive impact on the efficiency and dynamics of citing published work (see. The Effect of Open Access).
