DESCRIPTION Of IMMUNE STATUS IN WOMEN WITH LATENT PAPILLOMAVIRUS CERVICAL INFECTION
DOI:
https://doi.org/10.11603/24116-4944.2017.1.7382Keywords:
immune status, latent virus infection, lymphocyte phenotyping.Abstract
The aim of the study – analysis of indices change characterizing the state if immunity in women with genital latent papillomavirus infection often combined with urogenital infection at the beginning of observation and in six months.Materials and Methods. Women of reproductive age (from 18 to 45, 1200 patients), who addressed the gynecological departments of Perinatal Center and Clinical Hospital №18 of Kyiv for the period from 2011 to 2016. The control group consisted of 15 healthy women. The study was conducted with the use of such methods as cytological, colposcopic, bacteriological PCR, phenotyping of peripheral blood lymphocytes and statistical methods.
Results and Discussion. Human DNA of papillomavirus (HPV) was detected in 210 (16.0 %) of 1200 women with no clinical or morphological signs of infection, and that was the evidence of the latent human papillomavirus infection (PVI). In 84 women of them monoinfection was diagnosed, and in 126 – combined HPV and urogenital infections (UGI) were detected. HPV infection and further disease development expectancy (elimination or persistence of the virus) is mainly dependent on cellular and humoral immunity factors. To evaluate the part of human immune system in latent PVI the phenotyping of peripheral blood lymphocytes using monoclonal antibodies and immunoglobulin (Ig) A, M, G rates assesment using radial immunodiffusion method at the onset and in 6 month were applied.
In 140 women transient PVI and in 70 women persistent PVI were detected. Some signs of secondary immune deficiency appeared in T-lymphocyte and T-helpers rates depletion concurrently with the rise of cytotoxic lymphocytes and natural killers (NK) accounts with the background humoral immunity activation were detected in women with latent cervical PVI. Concurrent genital UGI supported and sometimes even increased those features of immunoreactivity in PVI. Normalization of cellular and humoral immunity parameters was observed in women with transient PVI, and further persistence of HPV in cervix uteri brought to more pronounced suppression of cellular and humoral immune system components reactions (depletion of NK rates and immunoregulatory index, IgA rates).
Conclusions. Thus, in women with latent cervical PVI we identified some signs of secondary immunodeficiency, manifested in the reduction of the percentage of T cells and T helper cells, while increasing the number of cytotoxic lymphocytes and natural killer cells against a background of activation of humoral immunity.
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