INTESTINAL MICROBIOTA RESEARCH METHODS IN PREGNANT WOMEN
DOI:
https://doi.org/10.11603/24116-4944.2023.2.14339Keywords:
intestinal microbiocenosis, dysbiosis, pregnancy, diagnosisAbstract
The aim of the study – to provide a comparative assessment of sampling methods for the study of intestinal microbiota during pregnancy.
Materials and Methods. A bibliometric and content analysis of publications of the last 5 years dedicated to the problem of researching the composition of intestinal microbiocenoses in women of reproductive age and pregnant women was carried out. The information search was carried out in PubMed, EMBASE, OVID, CINAHL databases. In addition, a study of the microbiota of stool samples of 57 pregnant women was conducted, including 27 – with dietary and constitutional obesity of the I–II degree (the main group), 30 – practically healthy women of normotrophic status (BMI = 20–25 kg/m2) with a physiological course of pregnancy. The research was carried out in the certified laboratory "Synevo" (Odesa). Statistical processing of the obtained results was carried out by the method of variance analysis using the Statistica 14.0 software (TIBCO, USA).
Results and Discussion. Only part of the known methods of researching intestinal microbiocenoses can be used in pregnant women. Preference is given to minimally invasive methods that do not require complex bowel preparation and have a minimal risk of secondary contamination. Our own observations showed that in pregnant women with obesity, MK, which corresponds to the II degree of dysbiosis, occurs more often than in the control group – 33.3 % and 6.7 %, respectively (χ2=6.49 df=1 p=0.01). Cases of III degree dysbiosis were not detected in any pregnant woman.
Conclusions. 1. Among the modern methods of sampling for the study of intestinal microbiota in pregnant women, the most promising are aspiration of intestinal contents, microbrushing and the use of an "intelligent capsule". 2. In pregnant women with obesity, II degree of dysbiosis occurs more often than in controls (χ2=6.49 df=1 p=0.01).
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